CORE--Biomarker

临床和转化研究最困难的组成部分之一是监测疾病活动和深入了解疾病机制的任务。解决这些问题的一种行之有效的方法是测量来自人类和动物样本的生物标志物。该核心的目标是提供来自该SCCOR计划的各个项目的样品中的生物标志物的测量。为了实现这一目标，我们将为这些单独的项目提供统一的资源，用于大规模测量血浆，血清和尿液中的生物标志物。我们还将使用组织培养 开发将采用患者样品暴露于培养细胞并测量诱发反应的报告基因测定的方法。我们还将集中和组织各个项目的样本储存，以便在本项目期间随时可以使用任何新的或新兴的生物标志物进行分析。在该核心单元中测量生物标志物的主要方法是市售酶联免疫吸附测定法（ELISA）或放射免疫测定法（RIA）。由于某些检测试剂盒可能无法在市场上买到，因此该核心还将使用市售抗体生成内部ELISA和RIA方法。该核心单元将生成涵盖SCCOR计划所有项目的数据，因此，应为动物模型和人类疾病之间的比较提供共同基础。

One of the most difficult components of clinical and translational research is the task of monitoring disease activity and developing insights into the mechanisms of disease. One well established method of addressing these issues is the measurement of biomarkers from human and animal specimens. The goal of this Core is to provide for the measurement of biomarkers in samples derived from the individual projects of this SCCOR program. In order to accomplish this goal, we will offer these individual projects a uniform resource for the large-scale measurement of biomarkers in plasma, serum, and urine. We will also use tissue culture methods to develop reporter assays that will employ the exposure of patient samples to cultured cells and measuring the evoked response. We will also centralize and organize the storage of samples from the individual projects so that samples are readily available for assay with any new or emerging biomarkers that become available during the course of this project. The principal means of measuring biomarkers in this core unit will be commercially available enzyme-linked immunoabsorbant assays (ELISAs) or radioimmunoassays (RIAs). Because some assays may not be commercially available, this core will also generate in house ELISA and RIA methods using commercially-available antibodies. This core unit will generate data that spans all projects of the SCCOR program and thus, should provide a common basis for comparison between animal models and human disease.