RNA Interference Core

描述（由申请人提供）：该项目将解决INIA-西验证INIA候选基因在过量饮酒中的作用的需要。RNA干扰核心将为INIA-West研究者提供用于RNA干扰介导的候选基因敲低或过表达的病毒载体。该项目的具体目标是设计靶向感兴趣基因的短发夹RNA（shRNAs）并进行体外筛选，产生表达病毒的shRNAs，用于特定脑区或转基因动物中的基因沉默，产生过表达的病毒感兴趣的基因，并验证体内基因沉默或过表达的有效性。INIA-West RNA Interference Core开发的病毒工具对于了解特定基因在调节过量饮酒中的作用至关重要。 公共卫生相关性：INIA-West的目标是确定导致过量饮酒的特定大脑区域中发生的分子，细胞和行为变化。RNA干扰核心将通过提供必要的工具来研究导致过度饮酒的基因在大脑中的作用，为INIA研究人员实现这一目标提供支持。

DESCRIPTION (provided by applicant): This project will address the need of INIA-West for validating the role of INIA candidate genes in excessive drinking. The RNA Interference Core will provide viral vectors for RNA interference-mediated knockdown or over-expression of candidate genes to INIA-West Investigators. The specific aims of the project are to design short hairpin RNAs (shRNAs) targeting genes of interest and perform in vitro screening of shRNA efficacy, to produce virus expressing shRNAs for gene silencing in specific brain regions or in transgenic animals, to produce virus for over-expression of genes of interest, and to validate the effectiveness of gene silencing or over-expression in vivo. The viral tools developed by the INIA-West RNA Interference Core are crucial to understanding the role of specific genes in regulating excessive alcohol consumption. PUBLIC HEALTH RELEVANCE: The goal of INIA-West is to identify the molecular, cellular, and behavioral changes that occur in specific brain regions that result in excessive alcohol consumption. The RNA Interference Core will provide support to INIA Investigators in reaching this goal by providing the tools necessary to study the role of genes in the brain that contribute to excessive drinking.