Protein-Glycan Interaction Resource of the CFG

描述（由申请人提供）：聚糖微阵列的制备和可用性由NIGMS/NIH通过Glue-Grant-supported Consortium for Functional Glycomics （CFG）开发。该资源通过提供丰富的500多种固定化聚糖，用于与识别聚糖决定因子（聚糖结合蛋白或GBPs）的蛋白质相互作用的一般筛选，彻底改变了糖组学领域。在胶水资助机制下成功工作了10年之后，由于2011年7月CFG的资金到期，这是一份继续支持这一关键“遗留”资源的申请。在这里，我们建议将这一资源作为“CFG蛋白-聚糖相互作用资源”继续进行，其中CFG的两个关键核心，Core D和Core H将被结合起来，以提供广泛应用于生物医学研究界的聚糖微阵列。目前，有兴趣使用此资源的研究人员将向CFG网站提交申请，该网站将通过CFG生物信息学核心B的其他遗留资金进行维护。目前，由该领域著名科学家组成的指导委员会将根据科学重要性和可用资源定期审查和批准/拒绝调查员的资源。在获得批准后，研究人员将把样品送到埃默里大学医学院，在那里，蛋白质-聚糖相互作用资源将被安置在CFG核心H目前使用的空间中，并由理查德·d·卡明斯博士（PD/PI）协调。蛋白质-聚糖相互作用资源使用的聚糖微阵列将由Scripps研究所的James Paulson博士（PD/PI）协调的聚糖阵列生产资源提供，最初将是5.0版本，但可以扩展，因为该领域的研究人员可能会为该计划提供聚糖。甘聚糖微阵列分析将在Emory进行，完成的结果将提供给研究人员和CFG的遗留生物信息学，供公众发布和管理。该项目将确保在制备、利用和管理聚糖微阵列数据方面的最高水平的完整性和系统性卓越，并为广泛的研究界提供这一宝贵的资源。

DESCRIPTION (provided by applicant): The preparation and availability of glycan microarrays was developed by the NIGMS/NIH through a Glue-Grant-supported Consortium for Functional Glycomics (CFG). This resource has revolutionized the glycomics field through making available a rich assortment of over 500 immobilized glycans for general screening for interactions with proteins that recognize glycan determinants (glycan-binding proteins or GBPs). This is an application for continued support of this key "legacy" resource due to the expiration of funding for the CFG in July 2011, following successful work for 10 years under the Glue Grant mechanism. Here we propose to continue this resource as the "Protein-Glycan Interaction Resource of the CFG", in which two of the key cores of the CFG, Core D and Core H, will be combined to make available glycan microarrays for wide use by biomedical research community. As currently, investigators interested in using this resource will submit their request to the CFG Website that will be maintained through other legacy funding to the Bioinformatics Core B of the CFG. As currently, a Steering Committee of eminent scientists in the field will periodically review and approve/deny resources for investigators based on the scientific importance and resources available. Following approval, investigators will send samples to Emory University School of Medicine where the Protein-Glycan Interaction Resource will be housed in space currently used by Core H of the CFG and coordinated by Dr. Richard D. Cummings (PD/PI). The glycan microarrays used by the Protein-Glycan Interaction Resource will be provided by the Glycan Array Production Resource coordinated by Dr. James Paulson (PD/PI) at The Scripps Research Institute, and will initially be Version 5.0, but can be expanded as glycans may be contributed to the program by investigators in the field. Glycan microarray analyses will be conducted at Emory and completed results made available to the investigators and to the legacy bioinformatics of the CFG for public posting and curation. This program will ensure the highest level of integrity and systematic excellence in the preparation, utilization, and management of glycan microarray data, and make available to the broad research community this valuable resource. PUBLIC HEALTH RELEVANCE: Glycan microarrays are used by hundreds of different investigators that have accessed them through the NIGMS/NIH Glue-Grant-supported Consortium for Functional Glycomics (CFG). This is a request for legacy funding to continue making available the glycan microarrays and associated databases and bioinformatics capabilities for the broad research community. This resource is unique and not duplicated elsewhere.