Whole genome assembly resources for aquatic models of human disease

描述(由申请人提供)：对水生实验模型进行激动人心的探索将产生新的科学贡献，每个模型都进化出在极其多样化和紧张的物理和生物环境中生存的新方法，这是我们对本提案所列物种基因组测序和组装的首要争议。许多长期存在的水生研究模型在我们对人类疾病的认识历史上具有显著而崇高的地位。此外，这些模型的不同属性为实验设计提供了可塑性，并提供了点燃创造性探究之火所需的发现广度。这就是非哺乳动物和水生动物模型的历史作用。然而，在过去的十年里，基因组学的力量彻底改变了我们对人类疾病分子基础的理解。不幸的是，由于与基因组资源开发相关的高成本，这一进展仅限于少数流行的和已建立的动物模型。自然，在少数模式生物的基因组学能力初步上升的同时，对使用不拥有这种资源的物种的比较生物学科学研究的支持兴趣也降低了。此前，我们组织了一次名为“人类疾病的水生模型”的会议，聚集了一个广泛的水生研究人员社区，寻求获得一份反映特定物种生物学广泛多样性的物种清单。更重要的是，这次讨论的重点是选择能够提供探索间接反映特定人类疾病的生物学的手段的物种。通过这次讨论和会议前举行的基因组学研讨会，我们汇编了一份具有模式生物已被证明能力的物种的优先名单(表1)。在这里，我们描述了我们为九种水生物种生成基因组资源的计划，这将使科学家能够测试一系列广泛的人类疾病途径起源的新假说。利用这些提出的水生生物基因组来推进我们对人类疾病的理解的伟大承诺现在是有必要的。 公共卫生相关性(由申请者提供)：确实需要继续开发和理解人类疾病的非人类模型。人类疾病的自然病程可能需要数年时间才能表现出症状；为了克服这个问题，研究人员开发了实验上易于处理的模型，使用可以模仿疾病的生物体--在几个月到几年的时间里，并具有遗传力量。到目前为止，我们还没有确定利用水生物种所需的资源，这些水生物种具有模仿各种人类疾病的独特属性。

DESCRIPTION (provided by applicant): Novel scientific contributions that would come from the exciting exploration of aquatic experimental models that each have evolved new ways to survive within exceedingly varied and stressful physical and biological environments is our foremost contention for the sequencing and assembly of the genomes of the species listed in this proposal. Many long-standing aquatic research models have notable and noble places in the history of our understanding of human disease. In addition, distinct attributes of these models provided plasticity in experimental design and a breadth of discovery needed to light the fires of creative inquiry. Such has been the historical roles for non-mammalian and aquatic animal models. However, the power of genomics over the past decade has revolutionized our understanding of the molecular basis of human disease. Unfortunately, due to high costs associated with the development of genomic resources, this advance has been limited to only a handful of popular and established animal models. Naturally, concurrent with the initial rise of genomics capabilities for a few model organisms there came reduced interest in support for comparative biological scientific inquiry that employed species that did not possess such resources. Previously we had organized a meeting, entitled "Aquatic Models for Human Disease", to gather a broad community of aquatic researchers seeking to gain input toward obtaining a list of species that reflect a broad diversity of species-specific biology. More importantly the focus of this discussion was to choose species that would provide a means to explore the biology that indirectly reflects a particular human disease. From this discussion and a genomics workshop held prior to the meeting, we have compiled a priority list of species with model organism proven capabilities (Table 1). Herein we describe our plans to generate genome resources for nine aquatic species that will empower scientists to test novel hypotheses for a wide array of human disease pathway origins. The great promise to forward our understanding of human disease using these proposed aquatic genomes is now warranted. PUBLIC HEALTH RELEVANCE (provided by applicant): There is a real need to continue to develop and understand non-human models of human disease. The natural course of a disease in a human may take years to manifest symptoms; to overcome this problem, investigators have developed experimentally tractable models employing organisms that can mimic a disease-in a period of months to a few years and with genetic power. We have thus far not established the resources needed to take advantage of the aquatic species having unique attributes that mimic a wide array of human diseases.