A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine.
A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine.
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DOI：
10.1038/srep34494
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发表时间：
2016-10-03
影响因子：
4.6
通讯作者：
McDonald MD
中科院分区：
文献类型：
--
作者：
Schauer KL;LeMoine CM;Pelin A;Corradi N;Warren WC;Grosell M;McDonald MD
海洋硬骨鱼在其肠道中产生CaCO3作为其肠道调节策略的一部分。这种沉淀对于地球上最大的脊椎动物群体的再水化和生存至关重要，但调节这种反应的分子机制尚不清楚。在这里，我们分离和表征的有机基质与海湾蟾鱼（Opsanus β）产生的肠道沉淀物。蟾鱼沉淀纯化使用两种不同的方法，并提取相关的有机基质。通过质谱和随后的数据库搜索，使用O.β转录组序列库。与肠液相比，许多鉴定的蛋白质在基质中富集，并且三种与NCBI数据库中任何先前表征的蛋白质没有实质性同源性。为了测试分离的基质的功能，设计了一种微改性的体外钙化测定，其显示低浓度的分离的基质显著促进CaCO3的产生，而高浓度显示出抑制作用。高浓度的基质也降低了镁的掺入到形成的矿物，可能提供了一个解释的镁含量的变化，在不同的鱼类产生的沉淀物中观察到的。
Marine teleost fish produce CaCO3 in their intestine as part of their osmoregulatory strategy. This precipitation is critical for rehydration and survival of the largest vertebrate group on earth, yet the molecular mechanisms that regulate this reaction are unknown. Here, we isolate and characterize an organic matrix associated with the intestinal precipitates produced by Gulf toadfish (Opsanus beta). Toadfish precipitates were purified using two different methods, and the associated organic matrix was extracted. Greater than 150 proteins were identified in the isolated matrix by mass spectrometry and subsequent database searching using an O. beta transcriptomic sequence library produced here. Many of the identified proteins were enriched in the matrix compared to the intestinal fluid, and three showed no substantial homology to any previously characterized protein in the NCBI database. To test the functionality of the isolated matrix, a micro-modified in vitro calcification assay was designed, which revealed that low concentrations of isolated matrix substantially promoted CaCO3 production, where high concentrations showed an inhibitory effect. High concentrations of matrix also decreased the incorporation of magnesium into the forming mineral, potentially providing an explanation for the variability in magnesium content observed in precipitates produced by different fish species.
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影响因子：
2.9
作者：
Alvares K
通讯作者：
Alvares K
影响因子：
5.8
作者：
Li, WZ;Jaroszewski, L;Godzik, A
通讯作者：
Godzik, A
影响因子：
3.6
作者：
Chikwati, Elvis M.;Gu, Jinni;Krogdahl, Ashild
通讯作者：
Krogdahl, Ashild
DOI：
10.1111/j.1432-1033.1982.tb05855.x
发表时间：
1982-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GRATAROLI, R;DIJKMAN, R;FIGARELLA, C
通讯作者：
FIGARELLA, C
影响因子：
1.6
作者：
GUNTHORPE, ME;SIKES, CS;WHEELER, AP
通讯作者：
WHEELER, AP