PROTEOMICS CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 蛋白质组学核心旨在通过结合二维凝胶、质谱学和Edman测序分析服务，提供定量和定性的蛋白质图谱和鉴定分析，以及翻译后修饰和序列分析。核心配备了用于二维凝胶分析的Bio-Rad IPG和SDS-PAGE仪器，用于检测和定量荧光蛋白染料(细胞染料)和染色(SYPRO-Ruby，Pro-Q Diamond)的台风磷成像仪/扫描仪系统，用于分析一维和二维凝胶的Ciphergen SELDI-TOF蛋白质图谱系统，具有MALDI和LC/ESI来源能力的ABI QSTAR II Q-TOF质谱仪，Waters高效液相色谱系统和多台PC，用于数据采集和分析，包括PDQuest软件的站点许可证和Ciphergen软件的5个站点许可证。训练有素的技术人员将协助调查人员提供全方位的蛋白质组学分析服务。工作人员将指导研究人员及其实验室工作人员(学生、博士后研究员和技术人员)进行样品制备、二维凝胶分析和荧光染色定量。比奇博士将对个别研究人员提供的样本进行MS/MS质谱仪和化学测序的蛋白质裂解、质量肽图谱和蛋白质测序。密码基因系统的样品制备和分析将由自动化机器人工作站提供便利。数据分析将由调查人员及其同事通过网络连接到密码基因系统的多个远程工作站进行。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The proteomics core is designed to provide quantitative and qualitative protein profiling and identification analysis, as well as post-translational modification and sequence analysis through a combination of 2D gel, mass spectrometry and Edman sequencing analysis services. The core is equipped with Bio-Rad IPG and SDS-PAGE apparatus for 2D gel analysis, a Typhoon phosphorimager/scanner system for detection and quantitation of fluorescent protein dyes (Cy-dyes) and stains (SYPRO-Ruby, Pro-Q Diamond) used to analyze 1D and 2D gels, a Ciphergen SELDI-TOF protein profiling system, an ABI QSTAR II Q-TOF Mass Spectrometer with both MALDI and LC/ESI source capabilities, Waters HPLC systems and multiple PCs for data acquisition and analysis including a site license for PDQuest software and a 5 station site license for Ciphergen software. Fully trained technical staff will assist investigators with the full range of proteomics analytical services. The staff will instruct investigators and their laboratory staff (students, post-doctoral fellows and technicians) in sample preparation, 2D gel analysis and quantitation by fluorescent staining methods. Protein fragmentation, mass peptide mapping and protein sequencing by both MS/MS mass spectrometry and chemical sequencing will be performed by Dr. Beach on samples provided by individual investigators. Preparation and analysis of samples with the Ciphergen system will be facilitated by automated robotic work stations. Data analysis will be performed by investigators and their colleagues through multiple distant work stations connected to the Ciphergen system via the web.