SCREENING FOR NOTCH AND SNAIL MUTANTS, AND ROLES OF IGFBP-2

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这项拟议的试点项目寻求应用代谢方法来发现可能平衡祖细胞分配给骨骼和脂肪形成的因子的新角色(和作用机制)。其中一个因子是蜗牛家族中的转录抑制因子。第二种是胰岛素样生长因子(IGF)的辅助因子，即IGF结合蛋白-2(IGF-BP2)。作为初步结果，每种基因的条件性基因敲除(由DRS Gridley和Rosen完成)似乎显著影响脂肪生成和骨形态形成。对IGF-BP2和Snail作用的全球和机械性洞察都将通过代谢监测来推进。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This proposed pilot project seeks to apply metabolic approaches to uncover new roles (and action mechanisms) for factors that may balance progenitor cell apportioning towards bone vs fat formation. One factor is a transcriptional repressor within the Snail family. A second is a co-factor for insulin-like growth factor (IGF) as IGF-binding protein-2 (IGF-BP2). As preliminary results, the conditional knockout of each (as accomplished by Drs Gridley and Rosen) appears to markedly affect adipogenesis and bone morphogenesis. Both global, and mechanistic insight into IGF-BP2 and Snail actions stand to be advanced through metabolic monitoring.