The Role of Keratinocytes as Independent Thermosensors

皮肤角质形成细胞和/或支配皮肤的感觉神经元中的温度敏感通道 对加热或冷却的反应，并调节我们对环境温度的感知。我们发现有些人 基底层表皮细胞和毛囊基质角质形成细胞表达TRPML3通道。我们有 还观察到，这个通道的打开是为了响应温度的变化，从寒冷走向 皮肤的稳态温度。在这里，我们建议解决以下问题。(1)哪些上皮细胞 表达TRPML3通道，以及在表皮和毛囊上皮发育的哪个阶段表达这些通道 表达这些阳离子通道？(2)这些细胞有冷温温敏电流吗？ 由其内源性TRPML3通道介导的电流？(3)TRPML3消除是否影响毛囊或 表皮形态？这些研究旨在阐明TRPML3通道在表皮和 毛囊，并可能导致进一步研究皮肤，包括血管系统和分泌腺，如何检测 并对温度的变化做出反应。

Thermosensitive channels in skin keratinocytes and/or in sensory neurons innervating the skin open in response to heating or cooling and mediate our perception of ambient temperature. We have found that some basal epidermal cells, as well as matrix keratinocytes of the hair follicles, express the TRPML3 channel. We have also observed that this channel opens in response to changes in temperature that go from cold towards the homeostatic temperature of skin. Here we propose to address the following questions. (1) Which epithelial cells express TRPML3 channels, and at which stages of epidermal and folliclular epithelial development do these express these cation channels? (2) Do these cells display cold-to-warm thermosensitive currents and are these currents mediated by their endogenous TRPML3 channels? (3) Does TRPML3 elimination affect hair follicle or epidermal morphology? These studies aim at elucidating the function ofTRPML3 channels in the epidermis and hair follicle, and may lead to further studies of how the skin, including the vasculature and eccrine glands, detect and respond to changes in temperature.