CONFOCAL MICROSCOPY CORE

共焦核心设施的目标是提供最先进的仪器设备，用于检查 单个活细胞和组织，具有亚微米三维分辨率。该设施配备了蔡司LSM 510 激光扫描共焦显微镜配备多台激光器，提供多种激发线。一个 可调谐脉冲Thapphire激光器(700-900 nm)为双光子和三光子提供200个飞秒脉冲 单光子激发截面在紫外光和紫外光下的各种荧光团的激发 可见光光谱。倒置尼康日食TE2000-S荧光显微镜 变形器可以获取广场荧光、亮场和相衬图像。该设施 为癌症中心的成员以及更广泛的凯斯西储大学社区服务， 每周7天营业。使用该仪器的时间仅限预约，癌症中心成员 将始终优先于非成员。设施总监对运营负有全面责任 为核心用户提供活细胞、共焦和多光子方面的专业知识和咨询 成像。工厂经理对工厂的显微镜部件负有日常责任 在董事的监督下。咨询委员会每年召开会议，处理任何与 设施运营、新技术开发和政策制定。咨询委员会向 癌症中心高层领导委员会。核心从事研究生教学，由 为生物学家组织一个为期2学分的研究生课程，光学显微镜和成像。学生获益 在经验丰富的指导下，利用最先进的光学成像设备进行广泛的动手培训 学术讲师。总的来说，共焦显微镜核心将对以下方面产生重大和积极的影响 癌症中心的科学项目。此外，设施在不同部门和部门之间的使用情况 中心将促进个人调查人员之间的富有成效的互动，以促进新的合作。

The objective of the Confocal Core Facility is to provide state-of-the-art instrumentation for examination of single living cells and tissues with submicron 3-dimensional resolution. The facility houses a Zeiss LSM 510 laser scanning confocal microscope equipped with multiple lasers to provide a variety of excitation lines. A tunable pulsed ThSapphire laser (700-900 nm) provides 200 femtosecond pulses for two and three-photon excitation of a variety of fluorophores whose one-photon excitation cross sections are in the ultra violet and visible light spectrum. An inverted Nikon Eclipse TE2000-S fluorescence microscope controlled by MetaMorph allows acquisition of wide-field fluorescence, bright field, and phase contrast images. The facility serves members of the Cancer Center, as well as the broader Case Western Reserve University community, and is open 7 days per week. Time on the instrument is by reservation only, and Cancer Center members will always have priority over nonmembers. The facility director has overall responsibility for the operations of the facility and provides expertise and consultation to core users in live cell confocal and multiphoton imaging. The facility manager has day-to-day responsibility for the microscopy component of the facility under the supervision of the director. An advisory committee meets annually to address any issues with facility operations, new technology development, and setting of policy. The advisory committee reports to the Senior Leaders Committee of the Cancer Center. The core is engaged in graduate teaching by organizing a 2-credit graduate course, Optical Microscopy and Imaging for Biologists. Students gain extensive hands-on training with state-of-the-art equipment for optical imaging guided by experienced academic instructors. Collectively, the Confocal Microscopy Core will have a large and positive impact on the Cancer Center's scientific programs. In addition, the use of the facility among different departments and centers will foster productive interactions between individual investigators to promote new collaborations.