GENOMICS SHARED RESOURCE

基因组学共享资源自2002年以来一直作为核心设施运营。GSR是在 NCCC作为满足NCCC研究需求的资源，但已扩大其范围， 为整个达特茅斯研究界提供服务。《施政报告》的主要目标是：（1）实施 用于基因调控和表达的全球分析的最先进的高通量技术;（2） 为微阵列服务开发一个有竞争力的低成本结构，以促进NCCC的使用 研究人员;（3）开发新技术，以开拓基因组学的其他途径。服务目前 GSR提供的服务包括：（1）分析RNA样品，以确定质量、数量和纯度;（2） 实验设计咨询与生物统计学和生物信息学陷阱资源; (3)亲和素基因表达谱分析;（4）Agilent基因表达谱分析;（5）Agilent miRNA阵列;（6） 来自冷冻和FFPE样品的Agilent CGH阵列;和（7）Agilent CpG岛阵列。服务是为了 在未来一年内提供的新技术包括：ChIP芯片阵列、安捷伦基因组拼接阵列和SNP分析。 研究者的信息和订购可通过互动网站以电子方式获得 <http://dms.dartmouth.edu/dgml/>，成立于2006年。一些新技术 在GSR开发的包括使用微阵列来确定整体转录率，hnRNA 加工，细胞质周转率，多核糖体进入率，以及细胞核与细胞质的差异 稳定状态的RNA水平。在截至2007年的财政年度，14个NCCC成员及其实验室（ 达特茅斯共有24个实验室使用了GSR，服务价值为238，000美元（366，410美元的-65% 总收入）。GSR的总体目标是为NCCC研究人员提供最先进的技术 以尽可能低的成本为他们的癌症研究提供支持和服务，以便他们能够成功地 实现他们的研究目标。

The Genomics Shared Resource has operated since 2002 as a core facility. The GSR was developed within the NCCC as a resource to meet the research needs of the NCCC but has expanded its reach to provide services to the entire Dartmouth research community. The major objectives of the GSR are to: (1) implement state-of-the-art high-throughput technologies for the global analysis of gene regulation and expression; (2) develop a competitive low-cost structure for microarray services that would promote use by NCCC investigators; and (3) develop new technologies to exploit additional avenues in genomics. Services currently offered by the GSR include (1) analysis of RNA samples to determine quality, amount, and purity; (2) experimental design consultations in conjunction with the Biostatistics and Bioinformatics Snared Resources; (3) Affymetrix gene expression profiling; (4) Agilent gene expression profiling; (5) Agilent miRNA arrays; (6) Agilent CGH arrays from frozen and FFPE samples; and (7) Agilent CpG island arrays. Services that are to be offered in the next year include: ChlP-on-chip arrays, Agilent genome tiling arrays, and SNP analysis. Information and ordering by the investigator is available electronically via the interactive website <http://dms.dartmouth.edu/dgml/>, which was established in 2006. Some of the new technologies being developed at the GSR include the use of microarrays to determine global transcription rates, hnRNA processing, cytoplasmic turnover rates, polysome entry rates, and differential nuclear vs. cytoplasmic steady-state RNA levels. During the fiscal year ending in 2007,14 NCCC members and their laboratories (of a total of 24 laboratories at Dartmouth) used the GSR with services valued at $238,000 (-65% of $366,410 total revenues). The overall goal of the GSR is to provide NCCC researchers with state-of-the-art technical support and services for their cancer research at as low a cost as possible so that they can successfully achieve their research objectives.