Qiagen PyroMark Q96 MD Automated Pyrosequencing System

描述(由申请者提供)：本申请申请购买一套QIGEN PYROMARK Q96 MD自动焦磷酸测序系统，以支持10多个由NIH资助的项目，涉及弗雷德·哈钦森癌症研究中心(FHCRC)的研究人员。这些项目涉及癌症研究的重要领域，包括识别和验证有可能预测各种癌症和其他基于基因的疾病的易感性的基因类型和表观遗传标记。焦磷酸测序提供了一种准确的手段，以高通量的方式快速评估组织中突变或表观遗传改变的等位基因的比例。这些信息对于确定疾病过程中这些基因改变的生物学和临床相关性至关重要。目前，西雅图地区的研究人员还没有这样的工具或类似的能力来评估基因改变或多态和表观遗传改变。如果获奖，PyroMark Q96 MD将被安置在FHCRC基因组学共享资源中，该资源拥有操作、维护和管理仪器的专业知识，并建立了收费基础设施以确保长期支持。所要求的仪器将满足当前和未来的需求，支持FHCRC由美国国立卫生研究院资助的翻译研究。

DESCRIPTION (provided by applicant): This application requests funds to purchase a Qiagen PyroMark Q96 MD Automated Pyrosequencing System to support over 10 NIH-funded projects involving researchers at the Fred Hutchinson Cancer Research Center (FHCRC). These projects address important areas of cancer research that include the identification and validation of genotypic and epigenetic markers that have the potential to predict the predisposition to various cancers and other genetic-based diseases. Pyrosequencing offers an accurate means to rapidly assess the proportion of mutated or epigenetically altered alleles in tissues in a high throughput manner. This information is critical in determining the biological and clinical relevance of these gene alterations in disease processes. Currently, there is no such instrument or similar capabilities for assessing both genetic alterations or polymorphisms and epigenetic alterations available to researchers in the Seattle area. If awarded, the PyroMark Q96 MD would be housed in the FHCRC Genomics Shared Resource, where expertise exists to operate, maintain, and manage the instrument, and a fee-based infrastructure is in place to ensure long-term support. The requested instrument will address both immediate and future needs, supporting translational NIH-funded research at the FHCRC.