X8 Prospector for Macromolecular Crystallography

描述（由申请人提供）：这是一份S10申请，要求为布鲁克X8探矿者集成衍射系统以及牛津低温流提供资金，以取代印第安纳大学医学院生物化学和分子生物学系老化的系统。用户组由6个主要用户组成，其中4个是NIH资助的研究人员，有10个活跃的拨款，另外5个正在等待NIH拨款。此外，两名获得美国心脏协会和美国国家科学基金会国家级资助的优秀新教师，以及Michael J. Fox基金会是用户组的一部分。x射线晶体学是研究大分子结构的有力工具。这项仪器要求支持的研究将使我们更好地了解我们的目标蛋白在健康和疾病中所起的作用。我们的许多主要用户也参与了药物发现研究，这可能最终导致改进治疗方法，以治疗酗酒、心血管疾病、糖尿病、神经退行性疾病和癌症等疾病。新仪器将取代已有16年历史的H2R旋转阳极发生器，整合到我们现有的X射线晶体学设备中。我们设施的升级将使我们富有成效的研究人员在NIH的资助环境中保持竞争力，毫无疑问，这将导致我们对生物医学相关大分子功能的理解取得新的进展。

DESCRIPTION (provided by applicant): This S10 application requests funds for a Bruker X8 Prospector integrated diffraction system along with an Oxford cryostream to replace the aging system within the Department of Biochemistry and Molecular Biology at the Indiana University School of Medicine. The user group consists of 6 major users, 4 of whom are NIH funded investigators with 10 active grants and an additional 5 pending NIH grants. In addition, two new outstanding new faculty members with national-level funding from AHA and NSF, as well as the Michael J. Fox Foundation are part of the user group. X-ray crystallography is a powerful tool for the study of macromolecular structure. The research supported by this instrumentation request will lead to a better understanding of the roles our target proteins play in health and disease. Many of our major users are also involved in drug discovery research, which may ultimately lead to improved therapeutic approaches to treat diseases such as alcoholism, cardiovascular disease, diabetes, neurodegenerative disorders and cancer. The new instrumentation will be integrated into our existing X- ray crystallography facility by replacing the over 16 year-old H2R rotating anode generator. The upgrade of our facilities will allow our productive research faculty to remain competitive in the NIH funding environment and will undoubtedly lead to new advances in our understanding of the function of biomedically relevant macromolecules. PUBLIC HEALTH RELEVANCE: X-ray crystallography is a powerful tool for the study of macromolecular structure. The research supported by this instrumentation request will lead to a better understanding of the roles our target proteins play in health and disease. Many of our major users are also involved in drug discovery research which may ultimately lead to improved therapeutic approaches for diseases such as alcoholism, cardiovascular disease, diabetes and cancer.