High Throughput Screening

高通量筛选（NTS）是一种快速评估数十万人的能力的手段。 化学化合物影响生物系统，以加速医学研究。我们 提出一个HTS核心，其目标是使Gl SPORE调查人员能够发现化学工具， 促进对结直肠癌生理学的更好理解，并有可能帮助揭示新的靶点， 治疗机会。使用最先进的HTS技术并与Gl SPORE调查人员合作， 范德比尔特化学生物学研究所的高温超导装置已经开发并验证了与高温超导兼容的 旨在发现与以下相关的化学工具的测定：1）人结肠癌中的E-钙粘蛋白调节 细胞系SW 620; 2）轴蛋白和β-连环蛋白在新的体外测定系统中的稳定性;和3）p120在 调节Colo 205细胞系中的E-钙粘蛋白活性和粘附连接形成。我们目前的数据 证明我们有能力进行HTS检测，发现影响所述系统的化合物， 以上，我们详细说明了我们的计划，使用HTS的产品，以促进Gl孢子的目标。

High-throughput screening (NTS) is a means of rapidly assessing the ability of hundreds of thousands of chemical compounds to affect biological systems for the purpose of accelerating medical research. We propose an HTS core the goal of which is to enable Gl SPORE investigators to discover chemical tools to promote better understanding of colorectal cancer physiology and potentially to help reveal novel targets and therapeutic opportunities. Using state-of-the-art HTS technologies and working with Gl SPORE investigators, the Vanderbilt Institute of Chemical Biology's HTS facility has developed and validated HTS-compatible assays aimed at discovering chemical tools relevant to: 1) E-cadherin regulation in the human colon cancer cell line, SW620; 2) axin and (3-catenin stability in a novel in vitro assay system; and 3) p120's role in modulating E-cadherin activity and adherens junction formation in the Colo205 cell line. We present data demonstrating our ability to perform the HTS assays, discover compounds that affect the systems noted above, and we detail our plans to use the products of the HTS to further the aims of the Gl SPORE.