Animal Core

这个核心单元是所有研究人员使用的正常和高血压动物的中央资源 计划项目。核心人员负责动物的持续和最新评估 对所有项目的要求。他们确保动物的及时订购、交付和适当的安置。所有动物 手术(除一些死后组织切除外)由核心B人员进行和/或监督。这 包括DOCA-盐和S6c诱导的高血压的产生和适当的对照或假手术 大鼠，用于体外分析的大多数常规组织切除，以及用于慢性血流动力学的大鼠仪器 监控。项目中使用的所有动物都仔细可靠地测量了它们的血压 由核心B人员直接(植入动脉导管或无线电遥测发射器)或间接 (尾袖血压计的收缩压)在每个实验方案之前或作为实验方案的一部分。每天 畜牧业、健康监测和记录保存也是核心B人员的责任，在 密歇根州立大学大学实验动物资源(ULAR)工作人员协会。 核心B人员还负责开发和实施新的生理技术 评估大鼠的心血管功能。动物核心的一个主要优势是它促进了最 项目调查人员对动物的有效利用。我们的惯例是安排组织样本采集 允许至少两个，通常是三个或更多的调查人员从一只老鼠那里获得 需要取自同一动物的组织。我们的体内协议也旨在最大限度地增加数据量 从一只动物身上采集的。这是通过使用长期重复测量的 心血管参数，而不是在每个感兴趣的时间点研究单独的大鼠组。通常是在 慢性活体方案的结束，来自大鼠的组织样本被分配到一个或多个其他方案 调查人员。因此，通过仔细的计划和改进我们的方法，我们能够显著地 减少用于实现我们的科学目标的动物数量。然而，目前还不可能取代 动物实验是我们了解动静脉功能控制的努力的一部分。

This core unit serves as a central resource for normal and hypertensive animals used by all investigators in the Program Project. Core personnel are responsible for continuing and up-to-date assessment of animal requirements for all Projects. They ensure timely ordering, delivery, and proper housing of animals. All animal surgery (except some post-mortem tissue removal) is conducted and/or supervised by Core B personnel. This includes production of DOCA-salt and S6c-induced hypertension and appropriate control or sham-operated rats, most routine tissue removal for in vitro analysis, and instrumentation of rats for chronic hemodynamic monitoring. All animals used in the Program Project have their blood pressure carefully and reliably quantified by Core B personnel either directly (implanted arterial catheter or radiotelemetry transmitter) or indirectly (systolic pressure by tail-cuff sphygmomanometry) prior to or as part of each experimental protocol. Daily animal husbandry, health surveillance and record keeping also are the responsibility of Core B personnel, in association with the staff of University Laboratory Animals Resources (ULAR) of Michigan State University. Core B personnel also are responsible for developing and implementing new physiological techniques for evaluating cardiovascular function in rats. A major advantage of the Animal Core is that it facilitates the most efficient use of animals by Program Investigators. It is our usual practice to schedule tissue sample collection from an individual rat in such as way as to allow at least two, and often three or more, investigators to obtain needed tissues from the same animal. Our in vivo protocols also are designed to maximize the amount of data collected from a single animal. This is achieved through use of long-term repeated measurements of cardiovascular parameters, rather than studying separate groups of rats at each time point of interest. Often at the end of chronic in vivo protocols, tissue samples from the rats are distributed to one or more other Program investigators. Thus, through careful planning, and refinement of our methods, we are able to significantly reduce the number of animals used to address our scientific aims. However, it is not yet possible to replace animal experimentation as part of our efforts to understand the control of artery and vein function.