PATHOLOGY AND METABOLIC RESEARCH CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 病理学和代谢研究核心描述 该核心为COBRE研究人员提供以下服务：（1）测量小鼠全身代谢，（2）通过无线电遥测测量小鼠血压，（3）量化小鼠动脉粥样硬化，（4）评估组织病理学，（5）糖尿病测量。 对于全身代谢的测量，16个TSE LabMaster平台可用于量化小鼠的食物和水消耗量、体力活动和间接热量测定。 该系统由一名研究技术人员监督，他将小鼠放入室中，运行所有设备，并以Excel格式向研究人员提供数据。 将小鼠在系统中饲养3.5天用于测量。 已经设计了一个协议，要求使用该系统和调度的目的。 对于血压测量，COBRE研究者可使用8个带软件的DSI平台。 提供颈动脉导管和植入物植入手术技术培训。 对于动脉粥样硬化的量化，可以进行主动脉弓中动脉粥样硬化病变的正面量化培训。对于组织病理学，研究者提交组织样本进行石蜡包埋和切片以及基础染色。 由经过培训的技术人员处理所有组织制备，并为研究人员提供培训。 对于糖尿病，我们正在建立小鼠正常血糖高胰岛素钳夹技术。 该核心还为小鼠葡萄糖和胰岛素耐量试验的训练提供帮助。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pathology and Metabolic Research Core Description This core provides services to COBRE investigators for (1) measurement of whole body metabolism in mice, (2) measurement of blood pressure by radiotelemetry in mice, (3) quantification of atherosclerosis in mice, (4) assessment of tissue pathology, (5) diabetes measurements. For measurement of whole body metabolism, 16 TSE LabMaster Platforms are available to quantify food and water consumption, physical activity and indirect calorimetry in mice. The system is overseen by a research technician, who places mice in the chambers, runs all equipment, and provides data in excel format to investigators. Mice are housed in the system for 3.5 days for measurements. A protocol has been designed requesting use of the system and for scheduling purposes. For measurement of blood pressure, 8 DSI platforms with software are available to COBRE investigators. Training in surgical techniques for implantation of carotid artery catheters and implants is provided. For quantification of atherosclerosis, training is available for en face quantification of atherosclerotic lesions in aortic arch. For tissue pathology, investigators submit tissue samples for paraffin embedding and sectioning and basic stains. A trained technician handles all tissue preparation, and provides training to investigator personnel. For diabetes, we are establishing methods for euglycemic hyperinsulinemic clamp technology in mice. The core also provides assistance for training in performance of glucose and insulin tolerance tests in mice.