COBRE: U OF KANSAS MEDICAL CTR: MOLECULAR BIOLOGY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子生物学核心将为所有Cobre科学家提供技术支持、最先进的设备和必要的分子生物学试剂，以便以经济高效的方式进行需要分子生物学方法的实验。CORE提供和维护的设施包括一台用于DNA测序的ABI Prism 3100遗传分析仪、两台实时PCR机、一台Biomek FX分析工作站(液体分配器)、五台用于聚合酶链式反应的热循环仪，以及其他常用的分子生物学设备，如具有密度分析能力的成像系统、细菌培养箱和超速离心机。核心负责维护和培训研究人员使用这些设施。此外，该中心还维护探针、引子、抗体、细胞系、小鼠组织和质粒的库存数据库，供科布雷研究人员共享。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core will provide all COBRE scientists with technical support, state-of-the-art equipment, and essential molecular biology reagents, so that experiments that require a molecular biology approach can be carried out in a cost-effective way. The facilities provided and maintained by the Core include an ABI Prism 3100 Genetic Analyzer for DNA sequencing, two real-time PCR machines, a Biomek FX Assay Work Station (liquid dispenser), five thermocyclers for polymerase chain reaction, and other commonly used molecular biology equipment, such as an imaging system with the capability for densitometry analysis, bacteria incubators, and ultracentrifuges. The Core is responsible for maintaining and training researchers to use these facilities. In addition, the Core maintains inventory databases for probes, primers, antibodies, cell lines, mouse tissues, and plasmids, which can be shared by COBRE investigators.