VALIDATING NOVEL GENES IMPLICATED IN LEUKOCYTE CHEMOTAXIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 一种预测未知基因功能的方法已经开发出来，并在有限的一组基因上进行了测试。该方法需要对所有平台和所有基因的微阵列数据集进行全球分析。大约38%的人类基因没有已知的功能，然而其中大约800个基因有足够的共表达数据和与一个或多个已知伙伴的明确的共表达模式来预测功能。这些未知的基因中有许多是“中枢”基因，即与许多其他基因一起反应的基因，并发现了对免疫相关功能的强烈偏向。我们已经将这一列表缩小到拥有最多数据以支持功能预测的40个。我们建议使用siRNA敲除和体外测试来测试这些基因中的每一个是否参与细胞周期和免疫系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A method of predicting unknown gene function has been developed and tested on a limited set of genes. The method entails a global analysis of microarray datasets, across all platforms and for all genes. Approximately 38% of human genes have no known function, yet approximately 800 of these genes have enough co-expression data and clear patterns of co-expression with one or more known partners to predict function. Many of these unknown genes are "hub" genes, or genes that respond with many others and a strong bias towards immune-related functions was discovered. We have narrowed this list to the 40 with the most data to support functional predictions. We propose to test each of these genes for their putative involvement in both the cell cycle and the immune system using siRNA knockdowns and in vitro assays.