PROTEOMICS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 蛋白质组学核心基金的主要责任是支持INBRE研究人员和北达科他州研究界的研究努力。蛋白质组学核心设施将由一个已建立的质谱学实验室和一个蛋白质分离实验室组成。核心设施将由约翰·沙布博士领导，他在蛋白质质谱学和蛋白质组学方面拥有丰富的经验。核心设施经理将负责设施的日常运营。核心设施将以适中的费用提供以下服务：通过二维凝胶电泳(2D-PAGE)分离蛋白质、2D-凝胶的图像分析，以及通过质谱学对分离的蛋白质进行蛋白质鉴定和结构表征。除了这些服务，该设施还将为该州的本科生、研究生和博士后研究员提供教育培训课程。培训课程将包括2D-PAGE蛋白质分离、2D-Gel图像分析和质谱仪鉴定蛋白质。因此，蛋白质组学核心基金将为北达科他州的生物医学科学家和学生提供机会，将蛋白质组学的尖端技术应用于他们的研究项目，并让学生在当前的生物医学研究中学习这一重要技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The major responsibility of the Proteomics Core Facility is to support the research efforts of the INBRE investigators and the research community of the state of North Dakota. The Proteomics Core Facility will consist of an established mass spectrometry laboratory and a protein separation laboratory. The core facility will be directed by Dr. John Shabb who has extensive experience in protein mass spectrometry and proteomics. The core facility manager will be responsible for day-to-day operation of the facility. The core facility will offer the following services at moderate fees: protein separation by two-dimensional gel electrophoresis (2D-PAGE), image analysis of 2D-gels, and protein identification and structural characterization of the separated proteins by mass spectrometry. In addition to the services, the facility will offer educational training courses to undergraduate students, graduate students and post-doctoral fellows within the state. The training courses will include protein separation by 2D-PAGE, 2D-gel image analysis and protein identification by mass spectrometry. Thus, the Proteomics Core Facility will provide opportunities to biomedical scientists and students in North Dakota to employ the cutting edge technologies of proteomics into their research projects as well as to students to learn this important technology in the current biomedical research.