ISOLATION AND STRUCTURAL CHARACTERIZATION OF MARINE FUNGAL SIDEROPHORES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 背景：许多微生物产生低分子量的铁(III)特异性结合化合物，称为铁载体来竞争铁。微生物被认为能产生独特的铁载体结构，从而成功地与环境中的其他生物竞争铁。从陆地细菌和真菌以及从海洋细菌中分离出来的铁载体在生物医药和控制微生物生长方面显示出很好的前景。海洋真菌产生的铁载体还没有被表征，因此提供了一种新的生物活性化合物的未知来源。 假设：海洋来源的真菌产生具有新结构的铁载体。 方法：在铁限制的条件下，在纯培养条件下筛选海洋来源的真菌菌株以产生铁载体。铁载体的分离和结构表征使用氨基酸分析、脂肪酸分析、核磁共振、质谱分析和其他技术，视情况而定。 结果和未来方向：已经筛选出12个海洋真菌菌株产生铁载体；12个菌株中有11个发现产生铁载体。目前正在对三个菌株产生的铁载体进行结构分析。从一种南极白孢子虫菌株中分离到一种分泌的铁载体。从沙门色孢子菌05-001中分离到一个分泌型羟甲酸铁载体和两个细胞相关铁载体。从雷氏青霉ATCC42470中分离到一株分泌型铁载体。这些铁载体的质谱分析已经完成。目前的工作集中在提纯足够的物质来完成核磁共振分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Many microorganisms produce low-molecular-weight, iron(III) specific binding compounds called siderophores to compete for iron. Microbes are thought to produce unique siderophore structures to successfully compete for iron against other organisms in their environment. The siderophores isolated from terrestrial bacteria and fungi and from marine bacteria show promise as biomedical agents and for control of microbial growth. The siderophores produced by marine fungi have not been characterized and thus provide an unexplored source of novel bioactive compounds. Hypothesis: Marine-derived fungi produce siderophores with novel structures. Methods: Marine-derived fungal strains are screened for production of siderophores in pure cultures under conditions of iron limitation. Siderophores are isolated and structurally characterized using amino acid analysis, fatty acid analysis, NMR, mass spectrometry, and other techniques, as appropriate. Results and future directions: Twelve marine fungal strains have been screened for siderophore production; eleven of the twelve were found to produce siderophores. Structural analysis of siderophores produced by three strains is currently underway. One secreted siderophore has been isolated from an Antarctic strain of Leucosporidium. One secreted hydroxamate siderophore and two cell-associated siderophores have been isolated from Sporidiobolus salmonicolor 05-001. One secreted siderophore was isolated from Penicillium raistrickii ATCC42470. Mass spectrometric analysis of these siderophores has been completed. Current work focuses on purification of sufficient material to complete the NMR analyses.