COBRE: LSU: CELL BIOLOGY AND TISSUE CULTURE CORE RESOURCE MODULE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个核心模块有四个主要功能。它维持已建立的细胞系，并为研究人员提供他们实验工作所需的细胞。它运营着一个细胞培养基厨房；大多数常见的细胞培养基加杜尔贝科的平衡盐溶液是从商业上可以买到的粉末混合物中制备出来的，过滤器经过灭菌，无菌检查和储存供个人使用。它容纳并维护着一个液氮杜瓦瓶银行，用于储存细胞系。最后，它帮助Cobre参与者、研究生、博士后研究员、研究助理和哺乳动物细胞培养艺术方面的教师强调无菌技术，以确保进行无抗生素细胞培养的能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core module has four primary functions. It maintains established cell lines and provides investigators with the cells needed for their experimental work. It operates a cell culture media kitchen; most of the common cell culture media plus Dulbecco's balanced salt solution are prepared from commercially available powder mixtures, filter sterilized, sterility checked and stored for individual use. it houses and maintains a bank of liquid nitrogen dewars for the storage of cell lines. Finally, it assists COBRE participants, graduate students, post-doctoral felows, research associates and faculty in the art of mammalian cell culture with emphsis on sterile technique to ensure the ability of performing antibiotic-free cell culture.