COBRE: LSU: IMAGING CORE RESOURCE MODULE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的双光子激光扫描显微镜配备了双振镜激光扫描仪，每个扫描仪独立地引导来自超快激光的两束激光。两种扫描仪都在定制软件的控制下，允许同时光释放各种笼状化合物和相关信号的双光子成像以及电子记录。优异的空间分辨率、组织穿透性和低光毒性的组合使显微镜能够用于广泛的实验。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The two-photon laser-scanning microscope available in our facility is equipped with dual galvonometeric laser scanners with each of these scanners independently directing two beams from ultrafast lasers. Both scanners are under the control of custom software allowing for simultaneous photo-release of various caged compounds and two-photon imaging of associated signals as well as electrical recordings. The combination of excellent spatial resolution, tissue penetration and low phototoxicity allows the microscope to be used in a wide range of experiments.