Transgenic Core

BADERC转基因核心位于贝斯以色列女执事医疗中心（BIDMC）。中心提供胚胎显微注射服务；具体来说，通过微注射研究者衍生的转基因DNA构建物来制造转基因小鼠，以及通过微注射研究者衍生的靶向胚胎干细胞（ES细胞）来制造基因敲除小鼠或基因敲入小鼠（即锁定等位基因、点突变等）。在该资助的最后5年周期（2004-2009年）中，BADERC转基因核心提供了及时、具有成本效益的服务。总共进行了92个项目（注射DNA转基因49个项目，注射靶向ES细胞克隆40个项目，突变胚胎冷冻保存3个项目）。这一服务水平表明，与该赠款的前5年周期（1999-2004年）相比，活动增加了84%。这些服务提供给来自4个不同机构的15名不同的BADERC调查员。在此期间，BADERC的研究人员发表了43篇涉及利用BIDMC转基因设施产生的转基因小鼠的论文。此外，BADERC

The BADERC Transgenic Core is located at the Beth Israel Deaconess Medical Center (BIDMC). The Core performs embryo microinjection services; specifically, microinjection of investigator-derived transgenic DNA constructs, for the purpose of making transgenic mice, and also microinjection of investigator-derived targeted embryonic stem cells (ES cells), for the purpose of making gene knockout mice or gene knockin mice (i.e. loxed alleles, point mutations, etc.). In the last 5 year cycle of this grant (2004-2009), the BADERC Transgenic Core provided timely, cost-effective services. In total, 92 projects were performed (injection of DNA transgenes - 49 projects, injection of targeted ES cell clones - 40 projects, cyropreservation of mutant embryos - 3 projects). This level of services represents a 84% increase in activity over the previous 5 year cycle of this grant (1999-2004). These services were provided to 15 different BADERC investigators from 4 different institutions. During this period, BADERC investigators published 43 papers that involved the utilization of genetically altered mice generated by the BIDMC Transgenic Facility. In addition, BADERC investigators obtained 22 NIH-based grants that involved the generation of genetically engineered mice. In summary, the Transgenic Core has provided extensive, cost-effective services to a wide array of productive BADERC investigators.