COMPARATIVE PROTEOMIC INVESTIGATION OF ARDS AND SYSTEMIC INFLAMMATORY INJURY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 急性呼吸窘迫综合征(ARDS)是重症监护医学中导致发病率和死亡率的重要原因之一。它可能是许多疾病的后遗症，如机械通气、败血症和创伤。该综合征的特征是炎症反应，导致肺泡-毛细血管屏障破裂，导致液体和蛋白质从血液进入肺泡腔。炎症反应的确切机制仍不完全清楚。已经进行了大量的临床和实验试验，以提高对这种疾病状态的了解和评估可能的治疗方案。方法：这项拟议的研究侧重于评估ARDS动物模型中的肺泡II型细胞与对照样本的比较。经蛋白质标记定量、层析、过滤、电泳法分离后，采用LC-MS-MS质谱仪对两组患者的蛋白质组进行分析，以确定疾病的蛋白质标志物。通过免疫分析进一步评估含量的变化。到目前为止，不同潮气量的机械通气和肝脏缺血后的变化已经被评估和发表。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The acute respiratory distress syndrome (ARDS) is one of the most important causes for morbidity and mortality in intensive care medicine. It can be the sequel of numerous diseases like mechanical ventilation, sepsis and trauma. The syndrome is characterized by an inflammatory reaction that leads to a breakdown of the alveolar-capillary barrier, resulting in an influx of fluid and proteins from the blood into the alveolar space. The exact mechanism of the inflammatory reaction is still incompletely understood. Numerous clinical and experimental trials have been made in order to improve the understanding and evaluate possible treatment options of this disease state. METHODS: The proposed study focuses on the evaluation of alveloar type II cells from animal models of ARDS compared to control samples. After protein labeling for quantitative analysis, separation by chromatography, filtration and electrophoresis, LC-MS-MS mass spectrometry is used to analyze the proteome in both groups in order to identify protein markers of disease. Content changes are further evaluated by immunoassays. So far alterations after mechanical ventilation with different tidal volumes and liver ischemia have been evaluated and published.