PROTEOMICS OF AXOPLASMIC SIGNALING COMPLEXES IN HEALTHY AND INJURED NERVES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该合作项目涉及神经元细胞轴浆中信号复合物的蛋白质组学表征。两种类型的复合物正在研究中，那些重要的长距离通信沿着轴突，和那些参与本地化的反应。亲和和差异蛋白质组学结合先进的质谱方法正在使用。此外，质谱方法正在开发，以确定和建立新的神经元轴浆蛋白的翻译后修饰。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This collaborative project involves the proteomic characterization of signaling complexes in neuronal cell axoplasm. Two types of complexes are under investigation, those important for long distance communication along the axon, and those involved in localized responses. Both affinity and differential proteomics incorporating advanced mass spectrometry methods are being employed. In addition, mass spectrometric methods are being developed to identify and establish novel posttranslational modifications in neuronal axoplasm proteins.