METAL IDENTIFICATION IN LUNG TISSUE IN AUTOPSY CASES FROM MEXICO CITY RESIDENTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们实验室以前的工作表明，随着时间的推移，肺组织中金属含量增加，可能与大气中悬浮颗粒物污染增加有关。通过原子吸收光谱分析，这些金属在30年期间有所增加，分别是镍、镉、锰、铅、钴、锌和钒。这一次，我们想通过X射线荧光更详细地鉴定肺组织中金属的存在，并使用光束线2-3上的Micro-XANES来确定它们的存在。光束线6-2上的透射式X射线显微镜和3D断层扫描将确定粒子与金属一起沉积的特定区域。对金属的识别以及沉积位置的表征将有助于更好地了解粒子对组织可能造成的损害。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previous work in our laboratory had indicated an increase in metal content in lung tissue through time, possible related to an increase in suspended particle atmospheric pollution. The metals, which increased over a 30-year period, were Ni, Cd, Mn, Pb, Co Zn and V, as.determined by Atomic Absorption Spectrometry. This time we would like to identify with more detail the presence of metals in lung tissue by x-ray fluorescence and to speciate them using micro-XANES, on beamline 2-3. Transmission x-ray microscopy and 3D tomography on beamline 6-2 would identify the specific area in which the particles with the metals are deposited.The identification of the metals as well as characterization of the site of deposition will help to understand better the possible damage that particles are inducing to the tissue.