MOUSE ROD OUTER SEGMENT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 光转导是一种信号级联，其中光子吸收被转换成电流的变化。光转导级联的成员大量存在于视杆细胞的薄外节隔室中。这些蛋白质中的许多已经被结晶，并且已经推导出高分辨率结构。然而，目前尚不清楚这些蛋白质是如何组装的。 这个建议的目的是获得一个详细的了解在脊椎动物杆光感受器的信号复合物。Chen实验室已经制造了许多靶向删除这些蛋白质的转基因小鼠。这些小鼠的外节断层扫描可能有助于重建参与光转导的蛋白质复合物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phototransduction is a signaling cascade whereby photon absorption is converted into a change in electrical current. Members of the phototransduction cascade are abundantly present in the thin outer segment compartment of the rod photoreceptor. Many of these proteins have been crystallized and high resolution structures have been deduced. However, it is not known how these proteins are assembled. The objective of this proposal is to obtain a detailed understanding of the signaling complexes in the vertebrate rod photoreceptor. The Chen laboratory has made a number of transgenic mice with targeted deletion of these proteins. Tomography of outer segments from these mice may be helpful in the reconstruction of the protein complexes involved in phototransduction.