REGULATION OF MEMBRANE TRAFFICKING BY BAR/F-BAR DOMAIN-CONTAINING PROTEINS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞维持和改变其膜形状的能力对许多细胞过程至关重要。外周和跨膜蛋白已被鉴定为参与内吞作用和形成细胞器结构的膜重塑物，并且这些蛋白质中的突变已与各种神经和发育疾病状态相关联。质膜上囊泡的形成由感知或诱导膜弯曲的蛋白质、催化最终分裂步骤的酶如发动蛋白和肌动蛋白细胞骨架介导。膜曲率感应和诱导蛋白的主要类别含有BAR结构域或相关折叠（例如F-BAR）。在体外和细胞中，这些蛋白质促进膜的滋养，并参与内吞作用和膜运输。我们使用X射线晶体学、小角X射线散射和其他生物物理方法的组合研究BAR和含F-BAR的蛋白质的结构和功能。这些结果将揭示这些蛋白质的特定功能和调控模式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability of cells to maintain and change the shapes of their membranes is vital for many cellular processes. Peripheral and membrane-spanning proteins have been identified as membrane remodelers involved in endocytosis and in shaping organellar structures, and mutations in these proteins have been linked to various neurological and developmental disease states. The formation of vesicles at the plasma membrane is mediated by proteins that sense or induce membrane curvature, enzymes such as Dynamin that catalyze the final fission step, and the actin cytoskeleton. A major class of membrane curvature sensing and inducing proteins contain BAR domains or related folds (e.g. F-BAR). In vitro and in cells, these proteins facilitate the tubulation of membranes, and are involved in endocytosis and membrane trafficking. We study the structure and function of BAR and F-BAR containing proteins using a combination of X-ray crystallography, small angle X-Ray scattering and other biophysical approaches. The results will shed light on the specific functions and mode of regulation of these proteins.