CHARACTERIZATION OF THE DSIK3 PROTEIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在这项研究中，我们对转基因果蝇的SIK3，盐诱导激酶，免疫沉淀物进行了质谱学研究，以确定SIK3中的残基在饲养过程中在脂肪体中经历了磷酸化。这项研究很重要，因为后生动物通过从葡萄糖转移到脂肪燃烧来维持能量平衡，了解调控这一过程的途径将进一步加深我们对肥胖等人类疾病的理解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this study we performed mass spectrometry studies on SIK3, salt inducible kinase, immunoprecipitates from transgenic flies to identify residues in SIK3 that undergo phosphorylation in a fat body during feeding. This study is important as metazoans maintain energy balance by shifting from glucose to fat burning and understanding the pathways that regulate this process will further our understanding of human disease such as obesity.