IDENTIFICATION OF THE DROSOPHILA F-BOX PROTEIN PALLBEARER SUBSTRATE(S)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在所有多细胞生物体中，细胞凋亡的清除对于免疫反应的发展和分解是至关重要的。未能清除凋亡细胞会引发自身免疫性疾病，如狼疮和神经退化，如年龄相关性黄斑变性，这是最常见的失明原因。我们已经确定了一种新的果蝇F-Box蛋白，它在细胞凋亡清除中发挥作用(Silva等人，2007年)。豁免27(4)：585-96)。该F-Box蛋白与SkpA、Lin19共同作用形成具有E3连接酶活性的SCF复合体。这个项目的目标是将免疫共沉淀和质谱学结合起来，以确定潜在的承受者底物(S)，然后在我们的实验室进行进一步的表征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Apoptotic cell clearance is crucial to the development and the resolution of immune responses in all multicellular organisms. Failure to clear apoptotic cells can trigger autoimmune diseases such as Lupus, and neurodegeneration, such as age-related macular degeneration, the most frequent cause of blindness. We have identified a novel Drosophila F-Box protein that plays a role in apoptotic cell clearance (Silva et al, 2007. Immunity 27 (4): 585-96). This F-Box protein acts together with SkpA, Lin19 and Effete to form a SCF complex with E3 ligase activity. The goal of this project is to couple co-ImmunoPrecipitation and Mass Spectrometry to identify potential Pallbearer substrate(s), which will then be further characterized in our laboratory.