IDENTIFICATION OF CRTC2 INTERACTING PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 CRTC2是CREB的共同激活剂，在喂养过程中促进胰岛细胞的活性 通过葡萄糖和胃肠激素的作用。在静止状态下，CRTC2是 通过与14-3-3蛋白的相互作用隔离在细胞质中。在激活时 在CREB途径中，CRTC2是从14-3-3蛋白中释放出来的，并有可能 与一组新的蛋白质相互作用。这项研究的目的是进一步描述 CRTC2相互作用蛋白的质谱学研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. CRTC2 is a co-activator of CREB, which promotes pancreatic islet cell viability during feeding through the actions of glucose and gut hormones. Under resting conditions CRTC2 is sequestered in the cytoplasm through its interactions with 14-3-3 proteins. Upon activation of the CREB pathway, CRTC2 is released from the 14-3-3 proteins and has the potential to interact with a novel set of proteins. The purpose of this study is to further characterize the interaction proteins of CRTC2 using mass spectrometry.