MULTI CRYSTAL EXPERIMENTS WITH INSULIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 采用声学液滴喷射(ADE)技术，将396孔板上的2.5uL晶体浆液液滴转移到MiTeGen微网销上。将对多个均质浆液进行测试，以验证声学样品制备对不同大小、不同浓度、不同总体纯度和清洁度的R3胰岛素和P43212溶菌酶晶体是有效和无损的。所有测试条件都能产生精确体积的准确转移液滴。我们将展示转移的液滴产生未损坏且衍射良好的晶体，其衍射图和所产生的结构与ADE安装和手工安装的样品类似。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Acoustic droplet ejection (ADE) was used to transfer 2.5uL droplets of crystal slurries from 396 well plates onto MiteGen micro mesh pins. Multiple homogenous slurries will be tested to verify that acoustic specimen preparation is effective and non-damaging for R3 insulin and P43212 lysozyme crystals of different sizes, different concentrations, and different overall purity and cleanliness. All tested conditions yielded accurately transferred droplets of precise volumes. We will show that transferred droplets yield undamaged and well diffracting crystals, with diffraction patterns and the resulting structures comparable for ADE mounted and hand mounted specimens.