PATHWAYS OF CONFORMATIONAL TRANSITIONS CRUCIAL FOR SIGNALING, ENZYME CATALYSIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们详细研究了三种不同蛋白质通过蛋白质/蛋白质相互作用的构象转换机制，它们分别代表信号蛋白、酶和调节子。我们小组最近在这三个系统上进行的实验表明，它们在不同共存构象之间相互转换的能力是实现其生物学功能的关键。我们研究的目标是对这些构象变化进行定量建模，并在分子水平上了解与触发和伴随相互转化最相关的特定相互作用。这项拟议的工作由能源部资助(奖励编号：De-FG02-05ER15699)和霍华德·休斯医学院。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose a detailed investigation of the mechanism of conformational transition for three diverse proteins representing a signaling protein, an enzyme and a regulator through protein/protein interaction. Recent experiments conducted within our group on these three systems have shown that their ability of interconverting between different coexisting conformers is key to the accomplishment of their biological function. The goal of our investigations is to quantitatively model these conformational changes, and to understand at a molecular level the specific interactions most relevant in triggering and accompanying the interconversions. The proposed work is funded by the Dept of Energy (Award No. DE-FG02-05ER15699) and the Howard Hughes Medical Institute.