IMAGING NANOPARTICLES FOR DIAGNOSTIC & THERAPEUTIC USE IN MOUSE TISSUES BY XFM

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目的目的是测试TiO 2及其衍生物纳米颗粒（Co-Fe-TiO 2和Co-Fe-Au-TiO 2）纳米颗粒和纳米颗粒生物有机分子纳米复合物作为诊断和/或治疗载体的可行性。 我们之前在培养细胞中的实验已经表明，TiO 2-DNA纳米复合材料具有独特和新颖的生物化学性质，非常适合于生物医学用途（Paunesku等人。Nature Materials（2003）2（5）：343-346）。 此后，我们开发了几种新的纳米颗粒，并研究了诱导细胞摄取这种纳米颗粒/纳米复合材料的不同方法（图1）。 因此，我们现在准备在小鼠体内测试这些不同的纳米结构。 我们的总体目标可以分为两个目标：目标1）测试不同的TiO 2纳米颗粒衍生物的体内稳定性和分布目标2）测试不同的纳米颗粒涂层的体内稳定性，并确定其对体内分布的影响。在BioCAT光束线上，我们正在使用X射线荧光在不同的大表面积小鼠组织切片中研究钛，钴，铁，金和相关的“生物”元素：P，S，Ca，Zn的存在。 我们将纳米复合材料直接注射到肿瘤组织中，或静脉注射或腹腔注射。 我们随后分离不同的组织，冷冻并冷冻切片。 我们寻找Ti信号与纳米颗粒中存在的其他元素的荧光信号的重叠，并通过将这些信号与组织中“天然”存在的P、S、Zn和其他元素产生的信号进行比较来绘制它们在组织不同区域中的存在。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to test the feasibility of TiO2 and derivative nanoparticles (Co-Fe-TiO2 and Co-Fe-Au-TiO2) nanoparticles and nanoparticle bio-organic molecule nanocomposites as diagnostic and/or therapeutic vehicles. Our previous experiments in cultured cells have shown that TiO2-DNA nanocomposites have unique and novel biochemical properties that would be very suitable for biomedical uses (Paunesku, et al.. Nature Materials (2003) 2 (5):343-346). We have since developed several new nanoparticles, and we investigated different ways to induce cellular uptake of such nanoparticles/nanocomposites (Figure 1). Therefore, we are now poised to test these different nano-constructs in vivo, in mice. Our overall goals can be organized in two aims: Aim 1) Testing different nanoparticle derivatives of TiO2 for in vivo stability and distribution Aim 2) Testing different nanoparticle coatings for in vivo stability and establishing their effects on in vivo distribution. At the BioCAT beamline we are investigating presence of titanium, cobalt, iron, gold and relevant "biological" elements: P, S, Ca, Zn using X-ray fluorescence in different mouse tissue sections with large surface area. We inject the nanocomposites directly into tumor tissue or intravenously or intraperitoneally. We subsequently isolate different tissues, freeze and cryosection them. We look for overlap of Ti signal with fluorescence signals of other elements present in nanoparticles and map their presence in different areas of tissue by comparing these signals with the signals produced by P, S, Zn and other elements "naturally" present in tissues.