Enhancement of the Integrated Aquatic Biomedical Models Facility Core

描述（由申请人提供）：我们要求资金，以现代化的电力服务，应急电力，并取代饮食准备厨房在辛胡贝尔水产研究实验室（SARL）在俄勒冈州州立大学。17,000平方米英尺SARL成立于46年前，提供世界上其他任何地方都没有的能力来支持水生脊椎动物模型的生物医学研究和培训。人类生物学的水生脊椎动物模型正迅速成为生物医学研究的快速、经济和强大的研究平台。在其显着的研究成就中，SARL是完成有史以来在任何脊椎动物物种（约42，000鳟鱼）中进行的两项最大的超低剂量致癌研究的关键，并帮助确定了alfatoxin作为一种主要的人类致癌物质。该设施最近收到了NIH RC 4主任的赠款，以进一步开发新的机器人技术和自动化，使斑马鱼成为第一个能够进行高通量和高内容筛选的脊椎动物模式生物。最近为SARL购买的其他设备，包括自动化液体处理，机器人样品处理和分析以及高含量成像仪器，大大增加了研究纳米材料，药物和其他环境化学品对斑马鱼的药理学，安全性和毒理学影响的能力。SARL已经取得了许多里程碑，使其处于斑马鱼研究的最前沿。其中包括：“第一个也是唯一一个无特定病原体的斑马鱼设施，”第一个规定的斑马鱼饮食，“一个标准化的远系繁殖SPF“野生型”斑马鱼，”多种创新技术，自动化养殖和斑马鱼处理。SARL目前拥有24名研究人员和工作人员，并在2010年支持联邦研究的直接费用520万美元。SARL设施还支持许多学生，博士后和兽医在使用水生模型的培训。两个T32培训赠款是基于SARL设施的独特能力：兽医（DVM）获得博士学位的新培训补助金，以及俄勒冈州立大学36年的T32毒理学培训补助金。申请的资金将使一个为期七年的改造计划得以完成，以重建一个核心设施，支持有史以来最大的脊椎动物实验。所要求的应急电力系统和新的电力服务是保护鱼类健康和防止长期实验中断所必需的。为了保护设施中饲养的大量动物的食物供应，以及允许现场制备定制的饮食，有必要对饮食厨房进行改造。这将增加SARL的独特能力，以探测营养缺乏对斑马鱼的影响。

DESCRIPTION (provided by applicant): We request funds to modernize the electric service, the emergency electrical power, and replace the diet preparation kitchen at the Sinnhuber Aquatic Research Laboratory (SARL) at Oregon State University. The 17,000 sq. ft. SARL was built 46 years ago and provides capabilities found nowhere else in the world to support biomedical research and training in aquatic vertebrate models. Aquatic vertebrate models of human biology are rapidly emerging as a rapid, cost effective, and powerful research platform for biomedical studies. Among its notable research accomplishments, the SARL was essential for completing the two largest ultralow dosage carcinogenesis studies ever conducted in any vertebrate species (~42,000 trout) and helped identify alfatoxin as a major human carcinogen. The Facility has recently received an NIH RC4 Director's grant to further develop new robotics and automation to enable zebrafish to become the first vertebrate model organism capable of high throughput and high-content screening. Other equipment recently acquired for SARL, including automated liquid handling, robotic sample processing and analysis, and high content imaging instrumentation, has greatly added to the capacity for studying the pharmacologic, safety and toxicological effects of nanomaterials, drugs, and other environmental chemicals in zebrafish. SARL has achieved a number of milestones to place it in the forefront of zebrafish research. These include: "the first and only specific pathogen-free zebrafish facility," the first defined diet for zebrafish, "a standardized outbred SPF "wild-type" zebrafish," multiple innovative technologies to automate breeding and zebrafish handling. SARL currently houses 24 researchers plus staff and supports $5.2 million in direct costs of federal research in 2010. The SARL facility also supports the training of many students, post- docs and veterinarians in the use of aquatic models. Two T32 training grants are based upon the unique capabilities of the SARL facility: a new training grant for veterinarians (DVM) to earn the Ph.D., and the 36-year T32 Toxicology Training grant at OSU. The requested funds will enable the completion of a seven-year remodeling program to rebuild a core facility will supporting the largest vertebrate experiments ever conducted. The requested emergency power system and new electrical service is necessary to protect fish health and to prevent disruption of long-term experiments. The diet kitchen remodeling is necessary to protect the food supply for the large numbers of animals maintained in the facility as well as to allow custom-defined diets to be prepared on site. This will add to SARL's unique capabilities to probe the effects of nutritional deficiencies to be investigated in zebrafish.