Biolmaging Core

该核心将为项目1、2和3提供最先进的生物成像支持，J.Zeng，H.Gendelman， 和Y·佩尔西茨基。这项工作将包括定量磁共振成像(MRI)、磁共振波谱 核磁共振成像(MRSI)和单光子发射计算机断层扫描(SPECT)。两只7特斯拉小型动物 目前在内布拉斯加大学医学中心运行的核磁共振/S系统将提供定量的 啮齿类神经艾滋病动物的神经影像和超顺磁性氧化铁标记细胞示踪 HIV-1相关性痴呆(HAD)模型。One GammaMedica Ideas动物SPECT将用于 确定伽马发射体标记细胞或分子探针的生物分布，并提供极好的 对核磁共振细胞跟踪方法的补充。图像处理实验室已经开发出定制的 包含可见基准标记的MRI/SPECT动物保持器，以允许这两者的共同注册 医疗模式。这使得SPECT图像的解剖细节和MRI对细胞定量的验证成为可能。 生物成像核心方法还包括血脑屏障通透性的定量测绘(项目 3，Y.Persidsky)、定量动脉自旋标记灌注成像和定量质子磁共振成像(1H (项目2和3，H.Gendelman和Y.Persidsky)。此外，先进的成像和光谱技术 在核磁共振/S核心设施内已经开发出分析方法，以实现小鼠大脑的自动化 子成像和成像结果与组织学的配准/翘曲(所有项目)。这个核心还将 支持与HAD小胶质细胞激活相关的发育疗法。这些项目的长期目标是 工作是评估神经病理和细胞的生理学MRI和生化1H MRSI预测因子 神经艾滋病小鼠模型的迁移动力学(MRI和SPECT)。这将提供敏感和 疾病发生和/或进展的特定筛查，以及提供对 各种治疗干预措施。从这个岩心获得的结果将直接适用于 监测人类神经退行性疾病的进程。对核心的重大修改是 在焦点和设计上都进行了设计，以便它可以毫不含糊地提供新的数据，这些数据不容易 仅通过组织病理学检查。

This core will provide state-of-the-art bioimaging support for projects 1, 2, and 3, J. Zheng, H. Gendelman, and Y. Persidsky. The work will include quantitative magnetic resonance imaging (MRI), MR spectroscopic imaging (MRSI), and single photon emission computed tomography (SPECT). Two 7-Tesla small animal MRI/S systems, now operative at the University of Nebraska Medical Center, will provide quantitative neuroimaging and superparamagnetic iron oxide (SPIO) labeled cell tracking for rodent neuroAIDS animal models of HIV-1 associated dementia (HAD). One GammaMedica Ideas animal SPECT will be used in determining biodistribution of gamma emitter labeled cells or molecular probes and provides an excellent complement to MRI cell tracking methods. The image-processing laboratory has developed custom MRI/SPECT animal holders incorporating visible fiducial markers to allow coregistration of these two modalities. This allows anatomical details for SPECT images and validation of cell quantification by MRI. The bioimaging core methods also include quantitative mapping of blood-brain barrier permeability (project 3, Y. Persidsky), quantitative arterial spin labeled perfusion mapping, and quantitative proton MRSI (1H MRSI) (projects 2 and 3, H. Gendelman and Y. Persidsky). In addition, advanced imaging and spectroscopic analysis methods have been developed within the MRI/S core facility to allow automated mouse brain subimaging and coregistration/warping of imaging results with histology (all projects). This core will also support developmental therapeutics relevant to microglial activation in HAD. The long-term aims of these works are to assess the physiological MRI and biochemical 1H MRSI predictors of neuropathology and cell migration dynamics (MRI and SPECT) in mouse models of neuroAIDS. This will provide sensitive and specific screens of the onset and/or progression of disease as well as providing response kinetics to a variety of therapeutic interventions. The results obtained from this core will have direct applicability for monitoring the course of human neurodegenerative disorders. Significant modifications of the core were made in both focus and design so that it can unambiguously provide novel data that cannot readily be btained by histopathological examinations alone.