BIOBANK CORE

生物库核心将协助项目成员实验室积累和集中胰腺癌研究所急需的生物资源。我们将利用我们直接获得人类手术标本的优势，为P01研究人员提供在组织学（与实验病理学核心合作）和遗传水平上进行广泛表征的生物材料。核心将收集、启动、维护、存档和记录所有人类PDAC生物资源。这些材料包括从原发性和转移性人类胰腺癌标本和PDAC细胞系库中创建的连续传代异种移植物。异种移植物肿瘤和细胞系受到高分辨率aCGH和表达谱的影响，使研究人员能够检索到具有适当基因组谱的所需生物材料。总之，这些资源应该促进分子研究，并为进行实验研究提供标准化的细胞系统。核心生物库的成员在异种移植系统的实施测试方面拥有丰富的经验

The BioBank Core will assist project member laboratories in the accrual and centralization of biologic resources that are critically required for pancreatic cancer research. We will take advantage of our immediate access to human surgical specimens to provide P01 investigators with biological materials that have been subjected to extensive characterization at the histologic (in collaboration with the Experimental Pathology Core) and genetic levels. The Core will collect, initiate, maintain, archive and record all human PDAC biologic resources. These materials include serially passaged xenografts created from primary and metastatic human pancreatic cancer specimens and a repository of PDAC cell lines. The xenograft tumors and cell lines have been subjected to high-resolution aCGH and expression profiling, enabling investigators to retrieve needed biologic materials with an appropriate genomic profile. Together, these resources should facilitate molecular studies and provide standardized cellular systems for performing experimental studies. Members of the BioBank Core have extensive experience in implementation of xenograft systems for testing novel therapeutic approaches and for molecular and biological studies. The Core will interact closely with the projects of the P01 to provide the biological materials and aid in the design and interpretation of experiments employing the xenografts and primary cells. The Core will seek to ensure a high level of quality control of specimens and standardization of protocols to ensure the effective translation of results between P01 projects.