Mouse and Transplantation Core

小鼠和移植核心将全面负责培训 皮肤同种异体移植和监测移植物存活。它还将负责 在BSL 2/3级生物防护中对病毒处理的实验动物进行维持、治疗和手术操作。所有计划项目实验将使用饲养在VAF设施或限制进入的生物防护设施中的VAF动物，该设施旨在保持最高程度的清洁度。强调严格限制进入的生物防护措施将防止意外传染物造成的交叉污染和结果腐败。 动物核心单位的目标和职责如下： 通过向VAF和生物防护中的动物施用或培训人员正确施用试剂，包括病毒、抗CDI 54 mAb、其他单克隆抗体、细胞、TLR激动剂和在项目过程中开发的其他特定试剂。 通过制备造血“同步嵌合体”，这是一种动物模型系统，对于成功的 项目1-3。 从生物隔离中的动物中获取细胞、活检和尸检标本，用于组织学和流式细胞术分析。 动物中心人员将为所有项目执行或协助执行皮肤移植。 对实验动物进行血清学检测，以证明感染已经发生，并且没有交叉污染。 简而言之，小鼠和移植中心将为每个项目提供必要的协助和人员培训，管理和执行在规定的VAF设施和生物防护中严格控制感染条件下进行的体内研究。

The Mouse and Transplantation Core will have overall responsibilities for training all Projects in the transplantation of skin allografts and monitoring for graft survival. It will also have responsibility for maintenance, treatment, and surgical manipulation of virus treated experimental animals in BSL2/3 level biocontainment. All Program Project experiments will utilize VAF animals housed in a VAF facility or in a limited access biocontainment facility designed to maintain the highest degree of cleanliness. The emphasis on strict limited-access biocontainment will preclude cross-contamination and corruption of outcomes by unintended infectious agents. The objectives and responsibilities ofthe Animal Core Unit are as follows: By administering or training personnel in proper administration of reagents, including viruses, anti-CDI 54 mAb, other monoclonal antibodies, cells, TLR agonists, and other specific agents to be developed in the course ofthe Program to animals in VAF and in biocontainment. By preparing hematopoietic "synchimeras," an animal model system that is crucial for the success of Projects 1-3. ¿ Procurement of cells, biopsy and autopsy specimens for histological and flow cytometric analysis from animals in biocontainment. ¿ Animal Core personnel will perform or assist with performing skin grafts for all Projects. ¿ Serological testing of experimental animals to document that infection has occurred and that there is no cross-contamination. In brief, the Mouse and Transplantation Core will provide essential assistance and personnel training to each Project with the management and execution of in vivo studies conducted in defined VAF facilities and in strictly controlled infectious conditions in biocontainment.