Imaging and Antibodies

核心B的目标是提供成像服务、抗体和抗体生产服务 计划项目的各个组成部分。应其他私家侦探的要求，酒井博士将讨论 实验，设计适当的实验策略和方案，然后执行商定的 服务。 成像服务：透射电子显微镜(包括免疫定位和标准 超微结构成像)和共聚焦显微镜可用于所有项目。 抗体：应要求，将向各项目分发下列特性良好的抗体： 针对纤维蛋白-1、纤维蛋白-2、LTBP-1和LTBP-4的兔多克隆抗体 专门针对纤维蛋白和骨形态发生蛋白-7。这些抗体都适合与小鼠材料一起使用。在……里面 此外，Core还可以提供针对flbrins-1、纤毛素-2和纤毛素-3的单抗，以及针对 弹性蛋白，可与人、牛和禽类纤维蛋白反应。《核心》还制作并刻画了 抗人纤维蛋白-4的单抗。 定制抗体：可产生兔多克隆抗体或鼠单抗，在 与PIS项目协商。PIS项目将提供免疫原，并将提供一些援助 进行抗体筛选和鉴定。因为pSmad2/3抗体的商业来源 由于不可靠和不一致，我们将合成磷酸Smad多肽作为免疫原， 产生并鉴定我们自己可靠的pSmad2/3抗体。 重组蛋白：也可获得有限的重组蛋白供应。这些措施包括 重组纤维蛋白-1和纤维蛋白2多肽；重组LTBP-1、LTBP-2、LTBP-3和LTBP-4 多肽和重组BMP-7。

The objectives of Core B are to provide Imaging services, antibodies, and antibody production services to the individual components of the Program Project. At the request of the other Pis, Dr. Sakai will discuss experiments, devise appropriate experimental strategies and protocols, and then perform agreed upon services. Imaging Services: Transmission electron microscopy (including immunolocalization as well as standard ultrastructural imaging) and confocal microscopy are available to all of the Projects. Antibodies: The following well-characterized antibodies will be distributed to the Projects upon request: rabbit polyclonal antibodies specific for fibrillin-1, fibrillin-2, LTBP-1, and LTBP-4; monoclonal antibodies specific for fibrillin and for BMP-7. These antibodies are all suitable for use with mouse materials. In addition, the Core can provide monoclonal antibodies specific for flbrillin-1, fibrillin-2, and fibrillin-3, and for elastin, which react with human, bovine, and avian fibrillins. The Core has also produced and characterized monoclonal antibodies specific for human fibulin-4. Custom-made antibodies: Rabbit polyclonal or mouse monoclonal antibodies can be generated, in consultation with Project Pis. Project Pis will provide the immunogen and will also perform some assistance with antibody screening and characterization. Because commercial sources of pSmad 2/3 antibodies have been unreliable and inconsistent, we will synthesize phospho-Smad peptides to use as immunogens, generate and characterize our own reliable pSmad2/3 antibodies. Recombinant proteins: Limited supplies of recombinant proteins are also available. These include recombinant fibrillin-1 and fibrillin-2 polypeptides; recombinant LTBP-1, LTBP-2, LTBP-3 and LTBP-4 polypeptides; and recombinant BMP-7.