Flow Cytometry

流式细胞术共享资源为托马斯杰斐逊大学Kimmel癌症中心的成员提供最先进的细胞分选和分析能力。该共享资源位于Bluemle生命科学大楼6楼约600平方英尺的专用实验室空间，位于托马斯杰斐逊大学校园的中心位置。流式细胞仪共享资源配备一台Cytomation MoFlo细胞高速分选仪、一台Coutler EPICS Elite流式细胞仪 一台Coulter XL-MCL自动化分析细胞仪、一台FACS Calibur台式分析仪、一台FACScan台式分析仪、两台400工作站、一台Nikon荧光显微镜、一台30立方英尺4 º C冰箱和一台10立方英尺-20 ° C冰箱。所有设备均由专门的管理员/操作员维护和管理。最常用的应用是表面表型和分选、混合群体中单细胞水平的细胞内细胞因子检测、Ca[2+]动员动力学的单细胞真实的时间分析、细胞凋亡研究、细胞周期分析和细胞周期特定阶段活细胞的分选。这种共享资源允许以高灵敏度和准确性及时进行实验和数据分析。同行评审，资助癌症中心的研究人员获得优先使用这一共享资源。基于新服务能力的提高，癌症中心成员使用该资源的稳步增加以及新研究人员的招募，我们预计共享资源的使用将在不久的将来显着增加。总之，这种共享资源在协助癌症中心研究人员进行癌症生物学和治疗研究方面发挥着关键作用。

The Flow Cytometry Shared Resource provides state-of-the-art cell sorting and analysis capability to the members of the Kimmel Cancer Center at Thomas Jefferson University. This shared resource is located in a dedicated laboratory space of about 600 sq ft on the 6th floor of the Bluemle Life Sciences Building, centrally positioned on the Thomas Jefferson University campus. The Flow Cytometry Shared Resource is equiped with one Cytomation MoFlo cell high-speed sorter, one Coutler EPICS Elite flow cytometer capable of sorting, one Coulter XL-MCL automated analytical cytometer, one FACS Calibur Benchtop Analyzer, one FACScan Benchtop Analyzer, two Celeron 400 workstations, one Nikon fluorescence microscope, a 30 cu ft 4¿C refrigerator, and a 10 cu ft -20¿C freezer. All units are maintained and managed by a dedicated manager/operator. The most commonly used applications are for surface phenotyping and sorting, detection of intracellular cytokines at the single cell level in mixed populations, single cell real time analysis of Ca[2+] mobilization kinetics, studies of apoptosis, cell cycle analysis, and sorting of viable cells in specific stages of the cell cycle. This shared resource allows experimental and data analysis to be performed in a timely manner and with high sensitivity and accuracy. Peer-review, funded cancer center investigators receive priority for the use of this shared resource. Based on increased capabilities for new services, the steady increase in the use of this resource by cancer center members and the recruitment of new investigators, we project that shared resource usage will increase significantly in the near future. In summary, this shared resource plays a critical role in assisting cancer center investigators as they conduct research into the biology and treatment of cancer.