ENDOMETRIOSIS MODEL

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 子宫内膜异位症的病理定义是异位部位的子宫内膜组织，包括腺体和间质。最常见的症状是严重的盆腔疼痛和不孕，大约40%的患者会出现这种情况。尽管科学上取得了一些进步，但涉及子宫内膜异位症和不孕症的机制仍不清楚。目前还没有专门的、非侵入性的诊断生物标记物，也没有治愈子宫内膜异位症的方法。对于影响全世界数百万妇女(十分之一的妇女)的子宫内膜异位症，迫切需要进行更多的研究。非人灵长类动物，包括恒河猴，已经被强调为研究子宫内膜异位症和不孕症的最合适的模型，因为它们在遗传、生理和解剖学上与人类相似，而且它们同时发生自发性和诱发性子宫内膜异位症。 本研究的主要目的是在UPR-CPRC建立恒河猴子宫内膜异位症(Macaca Mulatta)的诱导模型，以用于研究，从而更好地了解这种神秘的妇科疾病及其症状所涉及的机制。由于实际和伦理原因，这些研究需要反复进行腹腔镜术，不能在女性身上进行。CPRC非常适合进行拟议的研究，因为它提供了以下途径：i)注释良好的雌性恒河猴种群；ii)在子宫内膜异位症和不孕症方面拥有丰富知识和专业知识的主要合作者；iii)强大的技术，如DNA微阵列、基因表达分析和免疫测试。拟议的翻译研究的结果包括确定疼痛和不孕这两个重要女性健康问题的生物标记物和未来的治疗目标。这项研究将允许将研究结果直接应用于患者，并将发现新的假设，然后可以在未来的人类研究中进行测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Endometriosis is pathologically defined as the presence of endometrial tissue, both glands and stroma, in ectopic locations. The most common symptoms are severe pelvic pain and infertility, which occurs in approximately 40% of the patients. Despite some advances in science, mechanisms involved in endometriosis and infertility remain unknown. There are no specific, non invasive diagnostic biomarkers and no cure for endometriosis. There is a great need for more research in endometriosis that impact millions of women (1 in 10 women) around the world. Non-human primates, including the rhesus macaques, have been highlighted as the most appropriate model to study endometriosis and infertility, due to genetic, physiological, and anatomical similarities to humans, and the fact that they develop both spontaneous and induced endometriosis. The main objective of the present study is to establish an induced model of endometriosis in rhesus monkeys (Macaca mulatta) at the UPR-CPRC to be used for investigations that will lead to better understanding of the mechanisms involved in this enigmatic gynecological condition and its symptoms. These studies, which involve repeated laparoscopies, cannot be performed in women due to practical and ethical reasons. The CPRC is uniquely well-suited for the conduction of the proposed studies since it provides access to: i) a population of well-annotated, female rhesus macaques; ii) key collaborators with ample knowledge and expertise in endometriosis and infertility; and iii) powerful technologies such as DNA microarrays, gene expression assays and immuno tests. The outcomes from the proposed translational studies include identification of biomarkers and future therapeutic targets for two important women health issues, pain and infertility. This study will allow the direct application of findings to patients, and will uncover new hypotheses that could then be tested in future studies to be conducted in humans.