FUNCTIONAL GENOMICS OF ENDOCRINE DISRUPTION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 对火蜥蜴内分泌干扰的生态学和功能基因组学的研究将有助于开发一种可用于人类环境健康政策的两栖ED生物系统。使用微阵列分析和随后的原位杂交可以识别与阿特拉津暴露相关的差异基因表达。当这种基因组学方法得到对一系列条件的生态操纵的补充时，它允许确定一组可能的假设，即什么条件导致ED介导的基因上调和下调。阿特拉津和雌激素对性成熟有明显的影响，但也与调节两栖动物变态的激素相互作用。 这项建议中描述的工作旨在测试一种假设，即环境中常见的两种内分泌干扰物(阿特拉津和雌激素)的作用将与幼体火蜥蜴经历的正常生态线索相互作用。这种EDs与生态相互作用的结果是，亚致死浓度的EDs对火蜥蜴幼体的死亡率、生长以及变态和性发育的影响将大于预期。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Examination of the ecology complemented with functional genomics of endocrine disruption in a salamander will allow development of an amphibian ED bioassy system that can be used in human environmental health policy. Use of microarray analysis and subsequent in situ hybridization can identify differential gene expression associated with atrazine exposure. When this genomics approach is complemented by ecological manipulations of a broad array of conditions, it allows for identification of a likely set of hypotheses for what conditions are resulting in ED-mediated up- and down-regulation of genes. Atrazine and estrogen have obvious effects on sexual maturation, but also interact with the hormones that regulate amphibian metamorphosis. The work described in this proposal is designed to test the hypothesis that the action of two endocrine disruptors (atrazine and estrogen) found commonly in the environment will interact with the normal ecological cues experienced by larval salamanders. The result of such interactions of EDs and ecology will be larger-than-expected effects of sub-lethal concentration of EDs on mortality, growth and metamorphic and sexual development of the larval salamanders.