Advanced cryobanking for propagation-recalcitrant and critically endangered plant species

该项目旨在推进养护顽固/受威胁植物物种的方法，以便在生物多样性高的地区实现最佳做法的生态恢复。特别是，在组织培养和冷冻保存的不同阶段，将调查冷冻和脱水对细胞膜的损害的性质，试图将有害影响降至最低。此外，还将评估影响低温存活率的代谢变化，以进一步了解氧化应激的作用和低温保存过程的毒性。最终，将开发改进的低温方案，以保持生态恢复关键物种长期冷藏库的完整性，例如在雷后区域。

This project aims to advance methods for the conservation of recalcitrant/threatened plant species to achieve best practice ecological restoration in areas of high biodiversity. In particular, the nature of freezing and dehydration damage to cell membranes at various stages of tissue culture and cryopreservation will be investigated to try to minimise deleterious effects. In addition, metabolic changes that affect cryogenic survival will be assessed to provide further insight into the role of oxidative stress and the toxicity of cryopreservation processes. Ultimately, improved cryogenic protocols will be developed to maintain the integrity of long-term cryobanks of key species for ecological restoration, such as in post-mined areas.