Assembly and Structure of Bacteriophage Lambda

摘要 这个项目试图发现和描述病毒组装的机制， 最终以接近原子的分辨率，并包括对 病毒衣壳成熟的动态和能量方面。待研究对象 包括噬菌体HK97头部，对衣壳特别感兴趣 共价键的组装、衣壳成熟及其调控机制 交联性.噬菌体波长尾巴，特别是长度测定 以及组装伴侣的作用；噬菌体长尾纤维，具有 对装配伴侣的作用和相互作用方式特别感兴趣； 以及一组衣壳异常大的噬菌体的衣壳， 尤其感兴趣的是如何准确确定大小的衣壳。这部作品 将增强我们对病毒生命周期和分子机器的理解。 正在研究的这组病毒具有许多组装和 与动物病毒，特别是包括疱疹病毒一起成熟，所以这些 研究有可能加强我们对人类病原体的了解。

SUMMARY This project seeks to discover and describe mechanisms of virus assembly, ultimately at near-atomic resolution and including an understanding of the dynamic and energetic aspects of virus capsid maturation. Objects to be studied include the head of bacteriophage HK97, with particular interest in capsid assembly, capsid maturation, and control and mechanism of covalent crosslinking; phage lambda tails, with particular interest in length determination and the role of assembly chaperones; phage lambda long tail fibers, with particular interest in the role and mode of interaction of an assembly chaperone; and the capsids of a group of phages with unusually large capsids, with particular interest in how large capsid sizes are determined accurately. This work will enhance our understanding of virus life cycles and of molecular machines. The group of viruses under study shares many of the features of its assembly and maturation with animal viruses, especially including Herpesvirus, so these studies have the potential to enhance our understanding of human pathogens.