STEM CELL CORE

该提案的目标是建立一个干细胞核心设施（核心C），将产生小鼠骨髓间充质干细胞（BMSC）和肌肉卫星细胞，并提供这些细胞参与计划项目的个人项目。 一个关键的组成部分，必不可少的成功所提出的计划项目是骨髓间充质干细胞的可用性，因为所有的项目都需要这些细胞。因此，生产优质电池的集中核心设施对于这个动态协调的计划项目的成功至关重要。该核心将通过降低成本，为研究程序提供质量控制，并加强多学科之间的合作，以通过综合方法实现更大的科学收益，从而使个体研究者的研究工作受益。此外，从集中设施生产和分配细胞将确保所有研究从相同谱系和背景的细胞开始。为了实现我们的既定目标，本提案将追求以下目标： 1.从C57 BL/6小鼠以及转基因小鼠（ob/ob、生长激素缺乏和IGF-1缺乏小鼠）中分离、鉴定和分配BMSC和肌肉卫星细胞，以供单独项目使用。 2.为细胞培养、分化检测和基因表达分析提供技术支持。 通过实现这些目标，这个核心将作为一个独特的资源，提供小鼠间充质干细胞和技术援助的个人项目，以及其他医学研究格鲁吉亚学院谁是有兴趣的研究与衰老，代谢性骨疾病，或干细胞研究。

The goal ofthis proposal is to establish a Stem Cell Core Facility (Core C) that will produce mouse bone marrow-derived mesenchymal stem cells (BMSCs) and muscle satellite cells and provide these cells to the individual projects involved in the Program Project. A key component essential to the success ofthe proposed Program Project is the availability of BMSCs because all projects require these cells. Thus, a centralized Core facility for the production of quality cells is crucial to the success of this dynamically coordinated Program Project. This Core will benefit the research efforts of individual investigator by reducing costs, providing quality control for study procedures, and enhancing collaborations among multiple disciplines to achieve greater scientific gain through an integrated approach. In addition, production and distribution of cells from a centralized facility will ensure that all studies start with cells ofthe same lineages and background. To achieve our stated goal, this proposal will pursue the following aims: 1. To isolate, characterize and distribute BMSCs and muscle satellite cells from C57BL/6 mice as well as from genetically modified mice (ob/ob, growth hormone deficient, and IGF-1 deficient mice) to individual projects for their use. 2. To provide technical support for cell culture, differentiation assays and analysis of gene expression. By achieving these objectives, this Core will function as a unique resource to provide mouse mesenchymal stem cells and technical assistance to the individual projects, as well as to other Medical College of Georgia investigators who are interested in studies related to aging, metabolic bone diseases, or stem cell research.