Re-wiring of gene expression in the meiotic state

描述(由申请人提供)：概述-这个项目探索的想法是，当一个细胞进入减数分裂状态时，它经历了基因表达程序的根本性重新连接。一种更保守的观点认为，减数分裂细胞与营养细胞大体相似，但表达额外的减数分裂特有基因。我们探索了一种更激进的观点，即即使在营养细胞和减数分裂细胞中表达的基因也以不同的方式表达，受减数分裂细胞中不同的转录因子调控。此外，与营养细胞相比，减数分裂细胞可能使用更大的机制来控制基因表达。简而言之，当细胞从营养状态分化到减数分裂状态时，它们的基因表达程序被广泛地重新连接。我们将研究这种重新连接和新的减数分裂特有的调控机制。前三个项目使用酿酒酵母，分别侧重于减数分裂早期、中期和晚期的调控机制。第四个项目使用S.pombe，重点研究新的减数分裂调控机制。 项目。 1.早期减数分裂计划中的基因表达连接(Futcher博士) 2.CDC7减数分裂开关和底物，以及Ndt80的激活(Hollingsworth博士) 3.减数分裂状态中的组蛋白修饰和染色质开关(Neiman和Sternglanz博士) 4.减数分裂基因调控的新机制：反义RNA和RNA加工。(Dr. Leatherwood) 研究项目将得到微阵列核心设施的支持，该设施将在所有四个项目中发挥关键作用。

DESCRIPTION (provided by applicant): Overview - This Program Project explores the idea that when a cell enters the meiotic state, it undergoes a radical re-wiring of gene expression programs. A more conservative view was that meiotic cells are broadly similar to vegetative cells, but express additional, meiosis-specific genes. We explore a more radical view in which even genes that are expressed in both vegetative and meiotic cells are expressed in a different fashion, regulated by different transcription factors in meiotic cells. Furthermore meiotic cells may use a larger repertoire of mechanisms to control gene expression than vegetative cells. In short, when cells differentiate from the vegetative state into the meiotic state, their gene expression programs are extensively re-wired. We will study this re-wiring, and novel, meiosis-specific regulatory mechanisms. The first three projects use S. cerevisiae, and focus on regulatory mechanisms in early, middle, and late meiosis, respectively. The fourth project uses S. pombe, and focuses on novel meiotic regulatory mechanisms. Projects. 1. Wiring of Gene Expression in the Early Meiotic Program (Dr. Futcher) 2. Meiotic Cdc7 switches and substrates, and the activation of Ndt80 (Dr. Hollingsworth) 3. Histone modification and Chromatin Switches in the Meiotic state (Drs. Neiman and Sternglanz) 4. Novel mechanisms of gene regulation in Meiosis: Antisense RNAs and RNA processing. (Dr. Leatherwood) The research Projects will be supported by a Microarray Core Facility, which will play a crucial role in all four projects.