Molecular Diagnostic Core

项目概要（见说明）：分子诊断核心将支持三项主要研究 项目，并直接与数据库和生物统计核心互动， 生物材料，将在研究项目的过程中收集。核心活动包括 应用现有的方法，通过常规方法鉴定寄生虫和蚊子的种类和品系， 显微镜，目前的分子诊断方法，并通过新兴的细胞生物学和DNA测序 技术.具体目标是： 1：创建人类血液样本和蚊子的ICEMR档案，以确定疟疾物种的特征 和菌株，并用于评价疟原虫属感染的免疫学参数和临床 疟疾 2：开发基于PCR的检测方法，以诊断人类研究参与者的疟原虫属感染， 蚊子 3：开发基于PCR的检测方法，以诊断人类研究参与者中疟原虫株的感染， 蚊子 4：与传播项目合作，开发基于聚合酶链反应的检测方法， 蚊种形成、血源处理及氯菊酯抗性监测 5：开发基于显微镜和PCR的检测方法，以诊断人体内疟原虫配子体阶段 研究参与者 6：基于先进的DNA测序策略评估和制定策略，以分析 疟原虫种感染和按蚊多样性使用多态性分布在 寄生虫和媒介物种的基因组 7：建设能力并向疟疾流行地区的伙伴机构实验室转让技术 分子诊断核心将由曾与PNGIMR合作的齐默尔曼教授指导 （Mueller）、瑞士热带研究所（Felger）和昆士兰大学（库珀和毕比）的疟疾流行病学研究， 蚊子生态>10年。他将得到I Felger的协助，I Felger现在在STI工作，以前是 PNGIMR。疟疾流行地区的能力建设和培训将得到持续的高水平 该团队致力于将具有成本效益的技术扩展到ICEMR覆盖的地区。

PROJECT SUMMARY (See instructions): The Molecular Diagnostics Core will support the three major research projects and interact directly with the Database and Biostatistics Core in the organization of an archive of biological material that will be collected during the course ofthe research projects. Core activities include the application of existing methods for identifying parasite and mosquito species and strains by conventional microscopy, current molecular diagnostic methods, and by newly emerging cell biology and DNA sequencing technology. The Specific Aims are to: 1: Create an ICEMR Archive of human blood samples and mosquitoes for characterizing malaria species and strains, and for evaluating immunological parameters of infection by Plasmodium species and clinical malaria 2: Develop PCR-based assays to diagnose Plasmodium species Infections of human study participants and mosquitoes 3: Develop PCR-based assays to diagnose Infection by Plasmodium strains in human study participants and mosquitoes 4: In collaboration with the Transmission Project, develop PCR-based assays to perform mosquito speciation, process mosquitoes for blood meal source and monitor permethrin resistance 5: Develop microscopy- and PCR-based assays to diagnose Plasmodium gametocyte stages in human study participants 6: Evaluate and develop strategies based on advanced DNA sequencing strategies to analyze complexity of Plasmodium species infections and Anopheles mosquito diversity using polymorphisms distributed across the parasite and vector species genomes 7: Build capacity and transfer technology to partner institution laboratories in malaria endemic sites The Molecular Diagnostics Core will be directed by Prof. Zimmerman who has worked with PNGIMR (Mueller), the Swiss Tropical Institute (Felger) and UQ (Cooper and Beebe) on malaria epidemiology and mosquito ecology for >10 years. He will be assisted by I Felger, now at STI and previously an employee of PNGIMR. Capacity building and training in the malaria endemic site will be assured by the continuing high commitment of this team to expanding cost effective technologies to the region covered by the ICEMR.