Rapid Pathogen Detection using Super-Sensitive Multiplexing Nanophotonic Probes

为了响应快速分子诊断的迫切需要，本项目旨在探索新的光子学和生物化学方法来识别DNA。它被固定在专有的发光纳米点上，这些纳米点具有单分子灵敏度和可调谐的光学特性。该项目旨在开发DNA探针的多路试剂库，以检测痕量DNA分子并在一次检测中识别多种病原体。这项创新旨在为当前行业标准的病原体检测测试创建一个混合聚合酶链反应（PCR）技术平台。该项目的成果旨在使基于DNA的病原体诊断能够在90分钟内完成，比目前的检测快4倍。

Responding to an urgent need to advance rapid molecular diagnostics, this project aims to explore new photonics and biochemistry approaches to DNA recognition. It is anchored on proprietary light-emitting nanodots which have single-molecule sensitivity in conjunction with tunable optical identities. The project aims to develop a multiplexing reagent library of DNA probes to sense trace DNA molecules and to recognise multiple pathogens in a single assay. This innovation aims to create a hybrid-Polymerase Chain Reaction (PCR) technology platform for current industry-standard pathogen detection tests. The outcomes of the project aim to enable DNA based pathogen diagnostics within 90 minutes, four times faster than the current tests.