I-Corps: Point-of-Care Microfluidic Device for Rapid Pathogen Detection

I-Corps：用于快速病原体检测的护理点微流体装置

• 批准号: 1953841
• 负责人: XiuJun Li
• 金额: $ 5万
• 依托单位: University of Texas at El Paso
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1953841&HistoricalAwards=false
• 关键词: Corps; Point; Care; Microfluidic; Device

The broader impact/commercial potential of this I-Corps project stems from the critical need for a low-cost point-of-care (POC) microdevice for rapid detection of pathogen infections, such as whooping cough, in low-resource settings. Whooping cough is the only vaccine-preventable infection that remains endemic in the US. Conventional pathogen detection methods either take a long time or require costly and bulky instruments, limiting their applications for low-resource settings. The new microfluidic device can provide low-cost, rapid and accurate detection of whooping cough at the point of care, enabling rapid and accurate POC detection of whooping cough in rural clinical settings and other venues. The microfluidic device may also be used to detect a variety of other pathogens, such as foodborne pathogens. This I-Corps project is primarily intended to commercialize the microdevice for POC detection of pathogens. The core technology is based on integrated DNA isothermal amplification on a low-cost paper/polymer hybrid microfluidic device. The instrument-free detection by the naked eye minimizes instrumentation requirements, while DNA amplification and 6 primers specific to the target ensure high detection sensitivity and high accuracy, respectively. These significant features allow for low-cost, rapid (45 min), and accurate POC detection of pathogens in low-source settings.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该 I-Corps 项目具有更广泛的影响/商业潜力，源于对低成本护理点 (POC) 微型设备的迫切需求，用于在资源匮乏的环境中快速检测百日咳等病原体感染。百日咳是美国仍然流行的唯一可通过疫苗预防的感染。传统的病原体检测方法要么需要很长时间，要么需要昂贵且笨重的仪器，限制了其在资源匮乏环境中的应用。新的微流控装置可以在护理点提供低成本、快速、准确的百日咳检测，从而能够在农村临床环境和其他场所对百日咳进行快速、准确的POC检测。 微流体装置还可用于检测多种其他病原体，例如食源性病原体。 该 I-Corps 项目主要旨在将用于病原体 POC 检测的微型设备商业化。核心技术基于低成本纸/聚合物混合微流体装置上的集成 DNA 等温扩增。肉眼免仪器检测最大限度地减少了仪器要求，而DNA扩增和针对目标的6个引物分别确保了高检测灵敏度和高精度。这些显着的功能允许在低源环境中对病原体进行低成本、快速（45 分钟）和准确的 POC 检测。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优点和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

A new method to amplify colorimetric signals of paper-based nanobiosensors for simple and sensitive pancreatic cancer biomarker detection

• DOI: 10.1039/d0an00704h
• 发表时间: 2020-08-07
• 期刊: ANALYST
• 影响因子: 4.2
• 作者: Prasad, K. Sudhakara;Abugalyon, Yousef;Li, XiuJun
• 通讯作者: Li, XiuJun

Facile Synthesis and Integration of Poly(vinyl alcohol) Sponge-Supported Metal Nanocatalysts on a Microfluidic Chip Enable a New Continuous Flow Multireactor Nanocatalysis Platform for High Efficiency and Reusability Catalysis

• DOI: 10.1021/acssuschemeng.2c02060
• 发表时间: 2022-07-29
• 期刊: ACS SUSTAINABLE CHEMISTRY & ENGINEERING
• 影响因子: 8.4
• 作者: Hu, Kaiqiang;Ma, Lei;Li, XiuJun
• 通讯作者: Li, XiuJun

Remotely tunable microfluidic platform driven by nanomaterial-mediated on-demand photothermal pumping

• DOI: 10.1039/d0lc00317d
• 发表时间: 2020-06-21
• 期刊: LAB ON A CHIP
• 影响因子: 6.1
• 作者: Fu, Guanglei;Zhou, Wan;Li, XiuJun
• 通讯作者: Li, XiuJun

Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers

用于生物标志物视觉定量检测的无探测器光热条形图微流控芯片 (PT-Chips)

• DOI: 10.1021/acs.analchem.1c01323
• 发表时间: 2021
• 期刊: Analytical Chemistry
• 影响因子: 7.4
• 作者: Zhou, Wan;Fu, Guanglei;Li, Xiujun
• 通讯作者: Li, Xiujun

One-Step Surface Modification to Graft DNA Codes on Paper: The Method, Mechanism, and Its Application

• DOI: 10.1021/acs.analchem.0c00317
• 发表时间: 2020-05-19
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Zhou，Wan;Feng，Mengli;Li，Xiujun
• 通讯作者: Li，Xiujun