TRANSGENIC MOUSE AND ANIMAL MANAGEMENT

动物管理和转基因小鼠核心的目标是提供转基因和指定的 该计划的三个项目中的每一个项目都将基因敲除动物培育成不同的遗传背景 项目。由于每个项目需要大量的MICE，而且由于一些 多个项目共享基因类型，以确保最佳质量控制，鼠标 动物的鉴定和畅通，动物的繁育和基因分型的功能是最好的 在一个核心内由一个致力于该功能的团队进行。此外，订购、收据、进入数据库、 来自NIA殖民地的老年和年轻费舍尔344的分布将由Core C管理。 C，这个团队将由施方凡博士领导，并由麻省理工学院托马斯·马丁博士共同领导 比较医学。范博士在转基因育种和基因分型方面拥有丰富的经验 老鼠。在核心C中建立了促进小鼠繁殖、断奶、纹身、尾巴活组织检查和 Southern印迹/聚合酶链式反应(Southern blotting/PCR)确定基因分型。多个质量控制到位，以确保一致性 通过以特定间隔对每个品系的代表性小鼠进行Southern、Northern、Western blotting， 以及指示的活性分析。马丁博士在老鼠的护理和使用方面有丰富的经验， 对我们殖民地的老年老鼠的护理和管理进行了全面的描述。核心C 将在计划项目的五年中每年为三个项目中的每一个项目提供服务。

The goal of the Animal Management and Transgenic Mouse Core is to provide transgenic and the indicated knockout animals bred into distinct genetic backgrounds to each of the three projects of this Program Project. Due to the extensive numbers of mice that are required for each project, and since some of the genotypes are shared across more than one project, in order to ensure optimal quality control, mouse identification and smooth flow of animals, the functions of breeding and genotyping of animals are best done within a core by a team dedicated to that function. Further, the ordering, receipt, entry into the database, and distribution of aged and young Fischer 344 from the NIA colonies will be managed by Core C. In Core C, this team will be led by Dr. Shi Fang Van, and co-lead by Dr. Thomas Martin of the Institute of Comparative Medicine. Dr. Van has extensive experience in the breeding and genotyping of transgenic mice. Mechanisms are in place in Core C to facilitate mouse breeding, weaning, tattooing, tail biopsy and Southern blotting/PCR to determine genotype. Multiple quality controls are in place to ensure consistency of the strains by Southern, Northern, Western blotting of representative mice per strain at specified intervals, along with activity assays as indicated. Dr. Martin has extensive experience in the care and use of mice and has designed a thorough description of the care and management of aged mouse in our colonies. Core C will serve each of the three projects during each of the five years of the Program Project.