Novel nano-mechanical platform to investigate therapeutic sub-cellular mechanical

描述（由申请人提供）：该项目寻求对开发新型机械响应材料系统的支持，以研究局部细胞水平机械扰动与细胞对化学-物理生物材料表面特性的响应之间的关系。该涂层基于磁致弹性材料，该材料可以远程设置为以预定的振幅和轮廓振动。我们假设局部的亚细胞机械刺激可以用来优化基质-细胞的相互作用，以提高长期稳定性 在组织-植入物界面。最终，我们希望使用这些涂层作为一个实时系统，用于调制和监测可植入生物材料的表面。初步实验表明，亚微米局部振动，如所提出的振动涂层产生的振动，可能会与定制的生物材料表面特性相结合，以选择性地控制细胞粘附和基因表达，以促进和保持适当的整合在植入物-组织界面。该建议的具体目标是表征小的局部振动、基底材料表面特性和伴随的细胞对施加的振动的响应之间的关系。 公共卫生相关性：到目前为止，还没有有效的技术来控制植入式器械中生物材料-组织界面的长期劣化。虽然这是一个密集研究的领域，有许多新的方法涉及涂层和表面处理，这些方法不允许原位调节治疗效果。通过开发本提案中概述的技术，有可能监测植入物行为以及影响局部机械和化学环境的变化。此外，这项建议的结果将进一步影响对局部振动阿萨治疗工具的理解。直到最近，更大振幅和频率的振动（超声）对细胞行为的治疗作用的机制才变得更加清晰。我们的焦点部位特异性方法可能会导致这些平台涂层在植入式生物材料中使用的新策略和应用。

DESCRIPTION (provided by applicant): This project seeks support for the development of a novel mechanically responsive material system to study the relationship between local cell-level mechanical perturbation and cell response to chemical-physical biomaterial surface properties. The coating is based on a magnetoelastic material that can be remotely set to vibrate at a predetermined amplitude and profile. We hypothesize that local sub-cellular mechanical stimulus can be used to optimize substrate-cellular interactions to improve the long-term stability at the tissue-implant interface. Ultimately we hope to use these coatings as a real-time system for modulation and monitoring the surface of implantable biomaterials. Preliminary experiments have indicated that submicron localized vibrations, such as those generated by the proposed vibration coating, could potentially be coupled with tailored biomaterial surface properties to selectively control cellular adhesion and gene expression to promote and maintain proper integration at the implant-tissue interface. The specific goal of this proposal is to characterize the relationship between small local vibrations, substrate material surface character, and concomitant cellular response to applied vibrations. PUBLIC HEALTH RELEVANCE: To date, there are no effective technologies for controlling the long-term deterioration of the biomaterial-tissue interface in implantable devices. Although this i an area of intense study with many novel approaches involving coatings and surface treatments, these approaches do not allow for in situ modulation of therapeutic effects. By developing the technology outlined in this proposal, it may be possible to monitor implant behavior as well as affect changes in the local mechanical and chemical environment. In addition, the results of this proposal will further impact the understanding of local vibrations asa therapeutic tool. Only recently has the mechanism of larger amplitude and frequency vibration (ultrasound) therapeutic effects on cell behavior become clearer. Our focal site-specific approach may lead to new strategies and applications for the use of these platform coatings in implantable biomaterials.